These include glucose fermentation test, hydrolysis of L-arginin (Kleven and Levisohn, 1996) and digitonin
sensitivity test (Power and Jordan, 1976).
Mollicute isolates were confirmed using a Dienes probe, and Mycoplasma genera were identified with a digitonin
sensitivity test (WHITFORD et al., 1994; RAZIN & TULLY, 1996).
Purified liver mitochondria (1 mg) were suspended in 50 mM Bis-Tris and 500 mM 6-aminocaproic acid (pH 7.0) and solubilized by adding digitonin
(detergent : protein ratio of 1 : 5).
, and protease inhibitor cocktail were purchased from Sigma-Aldrich (St.
In brief, J774A.1 cells were permeabilized with extraction buffer containing 50 [micro]g/ml digitonin
for 15 min and the cell lysates were centrifuged.
Chemical permeabilizing agents like saponin, digitonin
, filipin, [alpha]-solanin, [alpha]-tomatin or [beta]-escin selectively attack the plasma membrane of muscle fibers, leaving the organelles and the cytoskeleton intact.
Behavior of crayfish rhodopsin and metarhodopsin in digitonin
: The 510 and 562 nm "visual pigments" are artifacts.
Tetramethyl rhodamine methyl ester (TMRM) is suitable for cytofluorometric measurements of mitochondrial membrane potential in cells treated with digitonin
. Biosci Rep 19(1):27-34, https://doi.org/10.1023/A: 1020193906974.
(1998) depicted milk treatment with saponin and digitonin
at various concentrations and reported that removal of cholesterol from milk was not dependent upon the concentration of cholesterol removing agent.
Furthermore, they found that perturbing the outer membrane of mitochondria with digitonin
or other mitochondria-damaging molecules inhibits the uptake process.
Camptothecin (20 [micro]M) and digitonin
(80 [micro]M) were used as positive controls for apoptosis and necrosis, respectively.
After the supernatant was eliminated, the pellet was added with isolation buffer containing 0.1% digitonin