After that diluted the bacteria in concentration of experimental by used sterilized MRS broth is dilutant. The dilution of bacteria sprayed on basal diet with 0, 1 x [10.sup.7], 1 x [10.sup.8] and 1 x [10.sup.9] [cfug.sup.-1] diet that test diets were daily prepared.
We added samples to 10 x 75-mm glass tubes and diluted them 1:5 by using supplied steroid dilutant. We added [sup.125]I and anti-corticosterone antibody to each tube and incubated them for 2 h at room temperature.
Vortex apparatus is commonly used to mix the interest plant sample with the dilutant. It is applied for dissolution, namely, in aqueous environment and polar solvents, of samples of plants to yield a fluid and homogeneous solution subject to analysis.