Because quercetin glucuronides was dissolved in Dimethyl sulfoxide
(DMSO) for the whole experiment.
(100 mg (Elmiron) (t.i.d) hydroxyzine HCI 10-75 mg/day, $0.18 taken at bedtime (25 mg) amitriptyline 10-150 mg/day, $0.36 taken with dinner (50 mg) dimethyl sulfoxide
50 mL of 50% $6.28 [**] (DMSD) solution, injected once a week tolterodine 4 mg/day $2.80 (Detrol LA) oxybutynin 5-15 mg/day $2.69 (Ditropan XL) (10 mg) celecoxib 100-200 mg/day $1.49 (Celebrex) (100 mg) rofecoxib 12.5-25 mg/day $2.52 (Vioxx) phenazopyridine 200-300 mg $2.22 t.i.d.
They used a relatively high MgCl, concentration (2.0 mmol/L) without dimethyl sulfoxide
in 35 cycles.
In the PCR, 100-200 ng of DNA was added to 25 [micro]L of reaction mixture containing 75 mmol/L Tris-HCl, pH 9.0, 20 mmol/L ammonium sulfate, 0.1 mL/L Tween, 1.5 mmol/L Mg[Cl.sub.2], 500 nmol/L of each primer, 0.2 mmol/L dNTPs, 100 mL/L dimethyl sulfoxide
, and 0.6 units of Taq polymerase (Advance Biotechnology).
To this reaction vial, 30 [micro]L of cell supernatant, 30 [micro]L of NaB[H.sub.4] solution (4 mol/L in 0.1 mol/L NaOH and 300 mL/L dimethyl sulfoxide
), 10 [micro]L of dithioerythritol (1.67 mmol/L in 2 mmol/L EDTA), and 30 [micro]L of 1-octanol (to avoid foam formation) were added in one step.
To enhance genotyping and to prevent misclassification of heterozygous individuals, we added 50 mL/L dimethyl sulfoxide
to the reaction mixture .
The research used mouse leukemia cells that, when treated with dimethyl sulfoxide
(DMSO), differentiate into red blood cells.
In brief, cells (about 1-2 x [10.sup.5] cells/well) in 24-well culture plates containing 1 mL medium per well were exposed to various concentrations (0, 0.3, 1.0, 3.0, 10.0, and 30 [micro]M) of the triazine herbicides or their metabolites (Riedel-deHaen, Seelze, Germany) (see structures in Figure 1) dissolved in 1 [micro]L of dimethyl sulfoxide
PCR amplification was performed in 40 [micro]L volume, and the reaction mixture consisted of 300-500 ng of DNA, 0.625 mmol/L magnesium chloride, 50 mmol/L potassium chloride, 240 [micro]mol/L of each dNTP, 120 g/L dimethyl sulfoxide
, 20 pmol of each primer (one labeled with FAM), and 1.25 U of AmpliTaq (Perkin-Elmer).
This might be caused by the inclusion of 50 mL/L dimethyl sulfoxide
in our amplification mixture.
The RNA was denatured with 1 M glyoxal and 50% dimethyl sulfoxide
, electrophoresed on an agarose gel, and transferred to a Hybond-N nylon membrane (Amersham Pharmacia Biotech).