The supernatant was removed, and 150 ml/well of dimethylsulfoxide
(DMSO, Sigma-Aldrich, St.
Note: 4204, IRX194204; DBT, dibutyltin; DMSO, dimethylsulfoxide
; M, molar; h/mPPARg, human/mouse peroxisome proliferatoractivated receptor gamma; h/mRXR, human/mouse retinoid X receptor; ROSI, rosiglitazone; SEM, standard error of the mean; TBT, tributyltin.
To induce neuronal differentiation, the preinduction medium was eliminated, and the cells were rinsed with PBS and changed to a neuronal induction medium composed of DMEM, 1% dimethylsulfoxide
(DMSO), and 100 [micro]M butylated hydroxyanisole (BHA).
In this study, fibroblasts from MADB patients exhibited marked nuclear abnormalities and reduced proliferation that improved upon treatment with rapamycin and dimethylsulfoxide
but not with other agents, including farnesyl transferase inhibitors.
The intercalation of TAAs in graphite has been reported previously, and the electrochemical reduction method has been adopted in polar aprotic organic solvents such as 1,2 dimethoxyethane, dimethylsulfoxide
(DMSO), N,N-dimethylformamide (DMF), and propylene carbonate [23-25].
Then, MTT was removed and 150 [micro]l dimethylsulfoxide
(DMSO; Sigma, USA) was added to the wells.
[D.sub.2]O, DCl, dimethylsulfoxide
(DMSO), hexadeuterated dimethylsulfoxide
(DMSO-[d.sub.6]), and n-octanol were obtained from Sigma-Aldrich, while MeOH was from Merck.
S-ISL was dissolved in dimethylsulfoxide
(DMSO) to make a 10 mg/mL stock solution, which was further diluted to appropriate concentration with culture medium before each experiment.
Numerous pigmentation enhancing agents have been discovered, such as diacylglycerols, 3-isobutyl-1-methylxanthine (IBMX), and dimethylsulfoxide
Other materials like dimethylsulfoxide
(DMSO), dinitrochlorobenzene, cyclophosphamide injection, ether solvent, and phosphate buffer saline were obtained from the Department of Pharmacology and Toxicology, University of Veterinary and Animal Sciences, Lahore (Syed Abdul Qadir Jillani (Out Fall) Road, Lahore - Pakistan).
The cryoprotectant solution commonly used in dourado is the generic formula designed for South American migratory fish (Carolsfeld et al., 2003), consisting in mixing 10% (15 mL) DMSO (dimethylsulfoxide
), 5% (7.5 g) glucose, a chicken egg yolk and 135 mL distilled water, and defined in this study as the standard cryoprotectant solution CS.
In brief, explants were pre-cultured in 1 mg/L BA, 5% dimethylsulfoxide
(DMSO) and 2% mg/L proline containing MS medium at 4C for 1d and then treated with 40 min in PVS2 solution.