Ehrenstorfer, 91% purity) were prepared by dissolving the reagents in dimethylsulphoxide
(DMSO, MERCK, 99.9% analytical grade) and then diluting the solution in four different concentrations for each pesticide as treatments (1, 10, 100, 1000 [micro]g [L.sup.-1]).
(DMSO) was used as a solvent for all the compounds and as negative control.
N, N'-dimethylformamide (DMF; d = 0.94 g [cm.sup.-3] at 20[degrees]C), N-methylpyrrolidone (NMP), N, N'-dimethylacetamide (DMAc), tetrahydrofuran (THF) and dimethylsulphoxide
(DMSO) was distilled over barium oxide under reduced pressure.
Spectrophotometric determinations of chloroplastidic pigments in acetone, ethanol and dimethylsulphoxide
. Revista brasileira de Biociencias 11: 52-58.
All the synthesized compounds were prepared in dimethylsulphoxide
[sup.1]H spectra were run at 500 MHz, and [sup.13]C spectra were run at 125 MHz in deuterated dimethylsulphoxide
(DMSO-[d.sub.6]) using TMS as an internal standard.
The nuclear magnetic resonance 1H-NMR spectra of dialdehyde and their polythioamides were recorded on a Bruker ACF300 spectrometer, using dimethylsulphoxide
(DMSO) as a solvent and tetramethyl silane (TMS) as an internal reference.
MTOR: Mechanistic target of Rapamycin ER: Endoplasmic reticulum HEKs: Human epidermal keratinocytes NGF: Nerve growth factor VEGF: Vascular endothelial growth factor HPV: Human papillomavirus ULK1: Unc-51-like kinase 1 Rictor: Rapamycin-insensitive companion of mTOR protein LC3: Microtubule-associated protein 1 light chain 3 AO: Acridine orange UPR: Unfolded protein response PERK: Protein kinase-like endoplasmic reticulum kinase IRE1[alpha]: Inositol-requiring enzyme 1 a eIF2[alpha]: Eukaryotic initiation factor 2 a JNK: Jun-amino-terminal kinase PARP: Poly(ADP-ribose) polymerase CHOP: Transcription factor C/EBP homologous protein DMSO: Dimethylsulphoxide
PI: Propidium iodide.
The dry plant extracts were reconstituted in dimethylsulphoxide
(DMSO) (Sigma Aldrich[TM], SA) for all cell based assays or in acetone for any other assay.
In vitro, CaD has been shown to scavenge oxygen free radicals generated by reaction between xanthine and xanthine oxidase in absence or presence of DMSO (dimethylsulphoxide
) and iron chloride (Fe[Cl.sub.2]) in a dose dependent manner (human polymorphonuclear blood cells).
demonstrated that after vitrification/warming of human ovarian tissue using dimethylsulphoxide
(DMSO) and ethylene glycol (EG), the expression of gene related to folliculogenesis did not change (13).