Also found in: Thesaurus.
ThesaurusAntonymsRelated WordsSynonymsLegend:
Noun1.dscDNA - double-stranded cDNA
cDNA, complementary DNA - single-stranded DNA that is complementary to messenger RNA or DNA that has been synthesized from messenger RNA by reverse transcriptase
Based on WordNet 3.0, Farlex clipart collection. © 2003-2012 Princeton University, Farlex Inc.
Mentioned in ?
References in periodicals archive ?
About 5 pg total RNA from each sample was used for labeling and array hybridization following these steps: (1) Reverse transcription by Invitrogen Superscript ds-cDNA synthesis kit; (2) dscDNA labeling with NimbleGen one-color DNA labeling kit; (3) array hybridization using the NimbleGen Hybridization System, followed by washing with the NimbleGen wash buffer kit; (4) array scanning using the Axon GenePix 4000B microarray scanner (Molecular Devices Corporation).
To construct the cDNA library, 5'-ends of newly synthesized dscDNAs were ligated with EcoRI adapter DNA by DNA ligase, and the 3'-ends of the cDNA were trimmed by XhoI.