dscDNA


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Noun1.dscDNA - double-stranded cDNA
cDNA, complementary DNA - single-stranded DNA that is complementary to messenger RNA or DNA that has been synthesized from messenger RNA by reverse transcriptase
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About 5 pg total RNA from each sample was used for labeling and array hybridization following these steps: (1) Reverse transcription by Invitrogen Superscript ds-cDNA synthesis kit; (2) dscDNA labeling with NimbleGen one-color DNA labeling kit; (3) array hybridization using the NimbleGen Hybridization System, followed by washing with the NimbleGen wash buffer kit; (4) array scanning using the Axon GenePix 4000B microarray scanner (Molecular Devices Corporation).
Whole genome expression profiling and signal pathway screening of MSCs in ankylosing spondylitis
To construct the cDNA library, 5'-ends of newly synthesized dscDNAs were ligated with EcoRI adapter DNA by DNA ligase, and the 3'-ends of the cDNA were trimmed by XhoI.
cDNA microarray analysis of differential gene expression in boar testes during the prepubertal period

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