Following the completion of the ion exchange stage of processing, which will produce purified lithium chloride eluate
, the eluate
will then enter a lithium carbonate process followed by washing with deionized water, drying, and then being micronised using a jet mill.
By bioactivity-guided fractionation procedure, the 95% ethanol eluate
(1.4 Kg) displayed antimicrobial and antioxidant activities and was chromatographed on silica gel CC, eluting with a gradient of CHCl3-MeOH (100:0a100:5a100:10a100:15a100:20), to give five fractions (F1-F5).
The KRAS Mutation Test v2 used 75 [micro]L of the DNA eluate
directly without DNA quantification or dilution, and MiSeq analysis used 25 [micro]L.
The first 1.0 mL of eluate
, discarded and 5.0 mL of eluate
sent into the amber vial and kept in the refrigerator for analyses [16, 17].
After each elution period, 3 mL of the eluate
was loaded into a new sterile tube (Greiner, Frickenhausen, Germany) and 100 [micro]L of lymphocyte isolate containing 100 cells/[micro]L was introduced.
An ESwab (Becton Dickinson, Franklin Lakes, NJ) was submerged in each of the prepared dilutions, then placed into the ESwab transport medium (eluate
), and vortexed for 10 seconds.
Fractionated elution of a commercially available [sup.68]Ga/68Ge-generator (IGG100, Eckert & Ziegler Isotope Products, Berlin, Germany; nominal activity of 1850 MBq) with 0.1 M hydrochloric acid (HCl, Rotem Industries, Israel) was used to obtain [sup.68]Ga[Cl.sub.3] (gallium chloride, ~310 MBq) in 1 mL eluate
. For labelling 20 [micro]g (5-10 nmol) of conjugate (mono-, di-, or trimer) was mixed with 100-500 [micro]L eluate
(~30-160 MBq) and the pH was adjusted to 4.5 by adding 20 [micro]L of sodium acetate solution (1.14 M) per 100 [micro]L eluate
Fraction 6 (2.0 g, 40% MeOH eluate
) was subjected successively to Sephadex LH-20 CC (50% MeOH), ODS CC (10% MeOH), and silica gel column (CH[Cl.sub.3]: MeOH : [H.sub.2]O = 8:2: 0.1) to obtain compound 4 (7.6 mg) and 11 (21.4 mg).
Caption: Figure 1: Absorbance at 280 nm of the eluate
from SP Sepharose Fast Flow column (10 ml each tube) containing a high concentration of protein.
(2015), DOC was observed to be rapidly released from biochar with no substantial amount of DOC recorded after a few eluate
The samples were passed through a chromatographic column and then the filtered eluate
was received, the collected extracts were dried under a gentle stream of pure nitrogen (N2).