Human dental stem cell derived transgene-free iPSCs generate functional neurons via embryoid
body-mediated and direct induction methods.
The new procedure is an advanced form of the embryonic stem cell test, in which substances are tested in vitro on so-called embryoid
3-dimensional cell culture for on-chip differentiation of stem cells in embryoid
Pfx (Invitrogen) was used to amplify gene open reading frames (ORFs) from 10-day Embryoid
Body cDNAs of H1 or cDNAs bought from YouBio (China).
body formation, 1 x [10.sup.6] hADSCs in 15 [micro]l DMEM/F12 supplemented with 2% B27, 20 ng/ml Human Epidermal Growth Factor (H-EGF), 20 ng/ml human basic Fibroblast Growth Factor (hbFGF) suspended on the inner side of tissue culture dishes were cultured for 48 hr under standard conditions.
(2007) found that undifferentiated cell clusters including embryonic stem (ES) cells, embryoid
bodies and mouse 11-day embryos had expression profiles of miRNAs that were simpler than those of mature somatic cells.
Characterization of embryoid
bodies of mouse embryonic stem cells formed under various culture conditions and estimation of differentiation status of such bodies.
It has been reported that hESCs first form embryoid
bodies which are basically structured with three germ layers.
Immunofluorescence of hiPSCs, embryoid
bodies (EBs), and hiPSC-derived DA neurons is described in Supplementary Materials and Methods.
Hybrid type N-glycan branches in a transition embryoid
body bear both a2,6- and a2,3- linked Sia in humans (61).
[D.sub.3] supplements effectively promoted osteoblast differentiation in mouse embryoid
body (EB) formation was initiated by harvesting HUES7 cells on feeder-free medium and transferring them to EB20 medium, which contains DMEM/F12 supplemented with 20% of fetal bovine serum albumin (BSA) (Sigma-Aldrich, Milan, Italy), 0.1 mM glutamine (Sigma-Aldrich, Milan, Italy), 0.1 mM nonessential amino acids (Gibco Invitrogen, Milan, Italy), 0.055 mM beta-mercaptoethanol (Gibco Invitrogen, Milan, Italy), 50 units/ml penicillin, 50 mg/ml streptomycin, and 50 [micro]g/ml of L-ascorbic acid (Sigma-Aldrich, Milan, Italy).