Moderate hyperplasia of the bronchial epithelium was present with increased numbers of goblet cells and a small number of embryonated
nematode eggs measuring 40 x 30 [micro]m within parabronchi and atria (Figs 2 and 3).
We isolated 6 EHDV in embryonated
chicken eggs, which consequently passed on BHK-21 cells, as previously described (6).
The methanolic extracts were made and concentrated by rotary evaporator and finally dissolved in distilled water before taking their antiviral trials in 7-11 days old chicken embryonated
The number of embryonated
eggs per well was counted using a stereo microscope (40x magnification).
Humans normally become infected by ingestion of embryonated
eggs (each containing a fully developed larva, L2) from contaminated sources (soil, fresh or unwashed vegetables, or improperly cooked paratenic hosts).
The mode of infection is by ingestion of embryonated
eggs in uncooked vegetables or water.
The nodular mass in oesophagus connects to oesophageal lumen through small orifices by which the female worm releases the characteristic ellipsoidal embryonated
The viruses were grown in the allantoic cavity of 10 day-old embryonated
Pilot studies are used to identify the relative suitability of differing cell-lines and embryonated
eggs from differing specific-pathogen free (SPF) chicken flocks by investigating dosing titers and incubation temperatures and times.
eggs in the blastopore closure stage were considered viable (GODINHO, 2007).
SPF fowl eggs were procured from Venky's (India) Ltd., SPF Eggs Division, Pune for propagation of virus as recommended by OIE, 2009.