Both explants (epicotyls
and internodal segments) were placed on Petri dishes containing 20 mL of MT culture medium supplemented with BAP (1.0 mg [dm.sup.-3]) and NAA (0.3 mg [dm.sup.-3]), or KIN (1.0 mg [dm.sup.-3]) and NAA (0.3 mg [dm.sup.-3]).
Growth of epicotyls
, turgor maintenance and osmotic adjustment in pea plants (Pisum sativum L.) subjected to water stress.
0.3 g of fresh seedling epicotyls
washed with deionized water, placed in tubes with 15 mL of deionized water and incubated for 2 h at 25degC.
Hypergravity increases the molecular Mass of Xyloglucans by Decreasing Xyloglucan-Degrading Activity in Azuki Bean Epicotyls
. Plant Cell Physiol.
Several kinds of explants including hypocotyls, cotyledon, leaf petiole, internodes and epicotyls
have been used for tomato cultivars (Gubiset al.,2003).
Each treatment had six replications for epicotyls
and eight for hypocotyls, with five explants per replication.
Merr.: plants from tissue-cultured epicotyls
," Plant Cell, Tissue and Organ Culture, vol.
After germination, seeds that germinated were counted, that is those which exhibit a breakthrough of epicotyls
and output of the first leaves.
Seedlings obtained from cytokinin treatments (24 mg L-1 BA) were stronger with thick hypocotyls, reduced epicotyls
and primary roots with few or no lateral root formation.
The roots, hypocotyls, epicotyls
, cotyledons and the eophylls were fixed in FAA 50 (formaldehyde, glacial acetic acid and ethyl alcohol) (JOHANSEN, 1940) and Glutaraldehyde (KARNOVSKY, 1965), embedded in historesin (GERRITS, 1991), cross-sectioned on a rotation microtome and stained with toluidine blue (O'BRIEN et al., 1964).
The expression of the CanVLEC gene was specific in seedlings, mostly in hooks and elongating epicotyls
, and no expression was detected in adult plants.
The first fully expanded leaves above the third node of the epicotyls
, or third node nomofile, were named seedlings with [+ or -] 10 cm in length, and then transplanted to plastic bags with a capacity of 5d[m.sup.3], containing a mixture of fertile soil texture vermiculite medium texture + coconut fiber medium texture + decomposed pine bark medium texture (1:1:1:1, v/v).