These results indicates that Integration of HPV16 more common than episome
genome in the host genome.
The genome of EqPV-H episome
is available in GenBank (accession no.
Lipkin, "Bocavirus episome
in infected human tissue contains Non- Identical termini," PLoS ONE, vol.
The linking regions of EBNA1 LR1 and LR2 were revealed to have a strong preference for G-quadruplex RNA and it was revealed that G-quadruplex RNA-interacting drugs block the functions of EBNA1 that are critical for viral DNA replication and episome
During latency, a limited number of viral genes are expressed which maintain the viral episome
These vectors are based on a well-established technology in which the inclusion of the EBNA1 gene and the OriP sequence of the Epstein-Barr virus allow a plasmid, after a single transfection, to replicate extrachromosomally, as a circular episome
, in several cell types .
Unlike HPV and HBV, EBV genome is not integrated into the host genome but maintained as an episome
inside latently infected cells [33, 35].
This approach is simple and fast and will greatly improve the way we study Leishmania genes compared to previous methods of gene tagging (e.g., by expressing GOI fused to a fluorescent tag from an episome
), since part of their endogenous regulation may be better maintained through the conservation of either the 5' or 3'UTR (depending whether the tagging is at the Cor N-terminus, resp.).
A genetic element based on synthetic nucleic acids capable of stable propagation, a synthetic episome
, is the minimal genetic element required for the systematic development of all cellular components of a synthetic organism based on artificial nucleic acids.
The cccDNA episome
is the transcriptional template for HBV messenger RNA transcripts that encode viral structural and NS proteins and the pregenomic RNA template for reverse transcription and synthesis of the viral genome .
In HPV-associated malignancies, high-risk HPV DNA may either be integrated within the cellular genome or it may be maintained as an episome
in the nuclei of the malignant cells.
As described previously , rKSHV.219 expresses enhanced green fluorescent protein (GFP) under the control of the strong cellular elongation factor 1-[alpha] promoter and the Ds-red fluorescent protein (RFP) from the KSHV lytic polyadenylated nuclear (PAN) RNA promoter, in addition to the gene for puromycin resistance as a selectable marker for maintenance of the viral episome
in rKSHV.219-infected cells .