exonuclease


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ex·o·nu·cle·ase

 (ĕk′sō-no͞o′klē-ās′, -āz′, -nyo͞o′-)
n.
Any of a group of enzymes that catalyze the hydrolysis of single nucleotides from the end of a DNA or RNA chain.

exonuclease

(ˌɛksəʊˈnjuːklɪˌeɪz)
n
(Biochemistry) an enzyme that is capable of detaching the terminal nucleotide from a nucleic acid chain. Compare endonuclease
ThesaurusAntonymsRelated WordsSynonymsLegend:
Noun1.exonuclease - a nuclease that releases one nucleotide at a time (serially) beginning at one of a nucleic acid
nuclease - general term for enzymes that catalyze the hydrolysis of nucleic acid by cleaving chains of nucleotides into smaller units
References in periodicals archive ?
DNA polymerase s and 8 exonuclease domain mutations in endometrial cancer.
The DNA polymerase used in pyrosequencing is the Klenow fragment (bacterial exonuclease I with the exonuclease function deleted).
The genomic DNA is then converted to single-stranded targets by using multiplex polymerase chain reaction (PCR) amplification and exonuclease digestion.
The probe is partially degraded by the polymerase's exonuclease activity in a template-specific reaction, and the product triggers the cleavage of a universal fluorogenic probe.
PCR amplifications were performed by using Ex Taq DNA polymerase (Takara Biomcdicals, Shiga, Japan), which has 3' [right arrow] 5' exonuclease activity.
Both pathways can occur in parallel, because Taq polymerases are known to possess different levels of exonuclease activity (22) and may dissociate hairpin structures with only partial digestion of the 5' terminus (23).
Mismatch repair is a complex process that depends on MMR proteins and multiple proteins that interact directly with DNA (single-stranded DNA-binding protein; exonucleases I, VII, and X; RecJ exonuclease; DNA helicase II; DNA polymerase III; and DNA ligase).
This probe contains a 2-kb EcoRI insert corresponding to a central region of SGI1, comprising parts of S023 and S024 open reading frames (ORFs), which code for putative helicase and exonuclease proteins (6).
It is a family-A DNA polymerase, with both DNA polymerase activity and exonuclease proofreading activity.
PCR products were purified with exonuclease I (New England Biolabs) and shrimp alkaline phosphatase (USB/Affymetrix) according to the manufacturers' instructions.
by using a real-time, 5' exonuclease (TaqMan) assay, based on a well-characterized polymerase chain reaction (PCR) assay that targets a highly-conserved 223-bp region of a gene (bcsp31) encoding a 31-kDa immunogenic B.
Lipid bilayers are formed at the top of the wells and nanopore constructs (a processive exonuclease enzyme combined with an a-hemolysin nanopore containing an adapter molecule, cyclodextrin) are added to the chip to sit within the lipid bilayers, forming a single hole in the bilayer.