exonuclease


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ex·o·nu·cle·ase

 (ĕk′sō-no͞o′klē-ās′, -āz′, -nyo͞o′-)
n.
Any of a group of enzymes that catalyze the hydrolysis of single nucleotides from the end of a DNA or RNA chain.

exonuclease

(ˌɛksəʊˈnjuːklɪˌeɪz)
n
(Biochemistry) an enzyme that is capable of detaching the terminal nucleotide from a nucleic acid chain. Compare endonuclease
ThesaurusAntonymsRelated WordsSynonymsLegend:
Noun1.exonuclease - a nuclease that releases one nucleotide at a time (serially) beginning at one of a nucleic acid
nuclease - general term for enzymes that catalyze the hydrolysis of nucleic acid by cleaving chains of nucleotides into smaller units
References in periodicals archive ?
Five ul PCR products were mixed with 1 ul exonuclease and 1ul alkaline phosphatase for purification.
The company said chemically modified RNA improves in vivo stability by protecting RNA molecules against exonuclease attack, allowing it to persist longer in certain cell Types and regions of genomic DNA that can be challenging to edit using conventional guide RNAs.
Trypanosome U-deletional RNA editing involves guide RNA-directed endonuclease cleavage, terminal U exonuclease, and RNA ligase activities.
PCR from 20 clones that exhibited a single amplified band of 500-1000 base pairs were cleaned with 16 units of Exonuclease I and 3 units of Antarctic Phosphatase (New England Biolabs) followed by ethanol precipitation.
smMIP capture was performed for 18 h overnight (10 min 95 [degrees]C, 18 h 60 [degrees]C, storage at 4 [degrees]C), followed by exonuclease treatment [0.
Mismatches can then be sensed and removed by action of the 3 to 5 exonuclease activity of the DNA Pol.
Furthermore, a subset of cases with "mixed serous and endometrioid carcinomas morphology" has been described in association with POLE exonuclease domain mutations, (22) a recently described genomic group by The Cancer Genome Atlas (6) with favorable clinical outcomes.
Lambda exonuclease was purchased from Thermofisher SCIENTIFIC (Canada).
There are several methods of producing single-stranded DNA molecules: asymmetric PCR [10], biotin-streptavidin separation [11], using exonuclease of phage [lambda] [12] and separating DNA strands during electrophoresis in polyacrylamide gel in denaturating conditions.
sup][60] established a new method detecting miRNA that was of high sensitivity by connecting LCR and fluorescence resonance energy transfer of cationic conjugated polymer assisted by lambda exonuclease.