KEYWORDS: Dental pulp, Tissue explant
, Mesenchymal stem cells, Permanent teeth, Primary/Deciduous teeth, Tissue engineering.
Plant regeneration through adventitious shoots and somatic embryos was achieved using different explant
types, basal medium or growth regulators on similar and close relative Muscari and Bellevalia species Bellevalia romana (Lupi et al., 1985), Muscari armeniacum Leichtlin ex Baker (Suzuki and Nakano, 2001), Muscari comosum var.
However, the selection of suitable types and sources of explant
are critical factors for obtaining a successful culture in somatic embryogenesis system (FENG & CHEN, 2014).
In this work, the regeneration or "shoots proliferation" from EAs was consistent with previous reports that concluded this type of explant
is regenerable but not optimum for the Agrobacterium-mediated genetic transformation and subsequent shoots production (Delgado-Sanchez et al., 2006; Arellano, Fuentes, Castillo-Espana, & Hernandez, 2009; Quintero-Jimenez, Espinosa-Huerta, AcostaGallegos, Guzman-Maldonado, & Mora-Aviles, 2010; Mukeshimana et al., 2013; Solis-Ramos et al., 2015).
The variables analyzed in the multiplication experiment were: number of shoots and leaves, explant
length and mean length of shoots and callus size (0 to 2), considering 0: no callus, 1: small callus (<1 cm) and 2: large callus (> 1 cm).
Analysis of variance was conducted on the evaluated traits to study embryogenesis in different explants
of chamomile at different levels of growth regulators (NAA) and kinetin Rooting Embryogenesis Degree of Sources of percentage percentage freedom variation 649.14 81.98 * 2 Replication 431.36 2133.91 *** 3 NAA 8401.05 *** 5397.64 *** 3 Kinetin 1927.78 * 936.11 * 2 Explant
1571.52 ** 1112.22 *** 9 NAA x Kinetin 1194.11 522.12 6 NAA x Explant
849.69 372.61 6 Kinetin x Explant
269.89 972.65 *** 18 NAAxKinetinxExplant 568.92 258.31 94 Experimental Error *, **And ***, indicate significant differences in the level of 5 percent, one percent and 0.1 percent.
The aim of this study is to identify the frequency of schistosomal liver fibrosis (SLF) in patients submitted to liver transplantation (LT) due to HCC in a reference center in Northeastern Brazil, describing the clinical, laboratory and histological characteristics of the patients whose explant
showed only SLF.
Somatic embryogenesis is a regenerative process of plants in which bipolar structures, similar to zygotic embryos, are formed by somatic cells with no vascular connection to the tissues of the initial explant
(Zimmerman, 1993; Jiang et al., 2012), making an asexual propagation system, especially for woody and palmaceous species considered difficult to regenerate by conventional asexual propagation methods (Kielse et al., 2007; Moura et al., 2008).
The authors observed up to 15.12 shoots per cotyledon explant
(STOJICIC et al., 2012).
(2004) for testing best explant
for plantlet regeneration.
Success of Agrobacterium-mediated transformation depends upon the regeneration frequency and number of shoots, which, in turn, depend upon the explant
types, pre-cultivation time, acetosyringone concentration, infection time and co-cultivation time.