(Eugene, Oregon) was applied to accentuate the fixed phagocyte ceils in the fluorescence microscope.
Using a fluorescent microsphere that excites and emits at one set of wavelengths, and a fluorescent nuclear stain that excites and emits at a different set of wavelengths, it was possible to image the microspheres in one channel and the fixed phagocyte cells in another channel.
The uptake of particles by large numbers of fixed phagocytes of the digestive gland cannot be analyzed in this way, as the manual technique for separating the terminal arterioles from the digestive gland tissue is laborious, and methods for separating the individual fixed phagocyte cells from the arterioles and perforated membranes are not available.
ABSTRACT The fixed phagocytes are noncirculating cells in the digestive gland (hepatopancreas) of the American lobster (Homarus americanus) and other decapod crustaceans.
KEY WORDS: Homarus americanus, American lobster, phagocytosis, fixed phagocytes, cellular immunity, fluorescence microscopy, confocal microscopy, Long Island Sound