Smears were stained with fluorescein isothiocyanate
(FITC) conjugate antihuman IgG, IgM, IgA and C3 for 30 minutes.
Light green color florescence emitted by the intraerythrocytic parasites under dark green background, observed with fluorescein isothiocyanate
(FITC) filter in a florescent microscope (Fig.
[alpha]GT, alpha-1,3- galactosyltransferase; KO, knockout; FITC, fluorescein isothiocyanate
Abbreviations: FITC, fluorescein isothiocyanate
; PE, phycoerythrin; SSC, side scatter.
Then, 250 [micro]L fluorescein isothiocyanate
(FITC) (500 [micro]g/mL in conjugation buffer) was added dropwise.
The researchers firstly produced colloidal gold nanoparticles, and then they modified them by using fluorescein isothiocyanate
3% polyvinylalcohol-(PVA-) water solution dyed with Rhodamine B and FITC (fluorescein isothiocyanate
) were used as sample liquids.
ERF values were also determined for microsphere calibrators for three other fluorescence channels: fluorescein isothiocyanate
(FITC), phycoerythrin (PE), and Pacific Blue(PB).
ABBREVIATIONS: AFB = acid-fast bacilli; CDC = Centers for Disease Control and Prevention; CSF = cerebrospinal fluid; DFA = direct fluorescent antibody; DNA = deoxyribonucleic acid; ELISA = enzyme-linked immunosorbent assay; FITC = fluorescein isothiocyanate
; GIS = Geographical Information System; H1N1 = influenza A virus; LRN = Laboratory Response Network; MDS = Molecular Detection Services; MGIT = mycobacteria growth indicator tube; MTB = Mycobacterium tuberculosis; PCR = polymerase chain reaction; PFGE = pulsed field gel electrophoresis; RNA = ribonucleic acid; STEC = shiga-toxin producing Escherichia coli; TB = tuberculosis; WHO = World Health Organization; WNV = West Nile virus
When macrophages were incubated with increasing glucose concentrations (5-40 mmol/L) for 18 h and analyzed by immunohistochemistry for CRP protein content, the intensity of fluorescein isothiocyanate
staining of the cells was proportional to the detected CRP concentration.
Cells were fixed and permeabilized using a commercial kit (Cytofix/ Cytoperm; PharMingen, San Diego, CA, USA) and stained for intracellular TNF-[alpha] using fluorescein isothiocyanate
(FITC)-conjugated rat anti-mouse TNF-[alpha] Ab (PharMingen).
Sites of probe hybridization were detected by applying 100 [micro]L of rabbit anti-biotin antibody (1:100 dilution with 2 mg/mL bovine serum albumin in 1x PBS) at 37 [degrees]C for 45 min, followed by using 100 [micro]L fluorescein isothiocyanate
(FITC) labeled goat anti-rabbit antibody (Enzo Diagnostics, New York; 1:100 dilution).