fluorescein isothiocyanate

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Related to fluorescein isothiocyanate: FITC
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Noun1.fluorescein isothiocyanate - a fluorochrome commonly conjugated with antibodies for use in indirect immunofluorescence
fluorochrome - any of various fluorescent substances used in fluorescence microscopy to stain specimens
References in periodicals archive ?
The researchers firstly produced colloidal gold nanoparticles, and then they modified them by using fluorescein isothiocyanate.
Key words: allophycocyanin (APC); equivalent reference fluorophores (ERF); fluorescein isothiocyanate (FITC); fluorescence; multicolor flow cytometer; Pacific Blue (PB); phycoerythrin (PE).
For FISH analyses, hybridization using vertebrate telomere peptide nucleic acid (PNA) probes (Telomere PNA FISH Kit/ fluorescein isothiocyanate [FITC]; DakoCytomation, Glostrup, Denmark) was performed according to the manufacturer's instructions with some modifications and as described in detail by Sakai et al.
10) Fluorescein isothiocyanate (FITC) conjugated antirabies antibodies are added directly to the slide and bind the rabies antigen if present.
When macrophages were incubated with increasing glucose concentrations (5-40 mmol/L) for 18 h and analyzed by immunohistochemistry for CRP protein content, the intensity of fluorescein isothiocyanate staining of the cells was proportional to the detected CRP concentration.
Quinine and harmine were non-covalently attached onto the surface of the starch nanoparticles, while gelatin nanoparticles were covalently labeled with fluorescein isothiocyanate.
Cells were fixed and permeabilized using a commercial kit (Cytofix/ Cytoperm; PharMingen, San Diego, CA, USA) and stained for intracellular TNF-[alpha] using fluorescein isothiocyanate (FITC)-conjugated rat anti-mouse TNF-[alpha] Ab (PharMingen).