At the appropriate growth stage, these non-blue wheats would be treated with a gametocide to induce male sterility and to encourage pollination by pollen from the untreated SB lines.
El-Ghazaly and Jensen (1990) and El-Ghazaly (1990) studied the effect of a gametocide on the development of the pollen wall and noted that the orbicules were smaller in size and with thinner walls than those in untreated anthers.