In the auto-incompatibility among flowers of the same plant, or geitonogamy
, flowers were bagged with paper bag and 20 flower buds in pre-anthesis were identified, and after full opening, flowers were crossed with each other using cotton-tipped stems and then bagged again.
The tests performed thereon were: spontaneous self-pollination, hand self-pollination (geitonogamy
), apomixis (by cutting the style with a forceps), cross-pollination, and open pollination (control).
); the close spacing of the plants are likewise allowed bees to forage on numerous individuals and facilitate crosspollination (i.e., xenogamy).
There were no significant differences in average number of matured pods per raceme, percentage of matured and aborted pods between bagged self pollinated flowers, cross pollinated flowers, Geitonogamy
, and open pollinated flowers.
The reproductive system experiment included four pollination treatments: autogamy (A), geitonogamy
(G), xenogamy (X), and open control (O).
As Wilson pointed out it appears pollination within the same flower (autogamy) and between flowers on the same plant (geitonogamy
) is extremely limited.
In such monoecious species, the spatial aggregation of clones creates a pollination environment that encourages geitonogamy
 by diminishing the size of an individual's pollen shadow and making it less likely that pollen disperses beyond the boundary of the genet.
--T4: Hand self-pollination (geitonogamy
)--a day before the work of pollination, 185 flower buds that would open the next day were bagged with tulle bag and were protected from any possible pollinator.
The presence of individuals with high level of similarity in one area may be possible, since with the use of co-dominant markers in physic nut and inbreeding have been observed in homozygous locus (SUN et al., 2008; BASH et al., 2009), assigned to the mode of propagation, geitonogamy
(pollination between different flowers of the same plant), as well as apomixis (PAIVA NETO et al., 2010).
The next day, the bags were removed and the stigmas were brushed with the freshly dehisced anthers from the flowers of the same tree and re-bagged to know whether fruit set occurs through geitonogamy
. Ten trees were selected for manual crosspollination and open-pollination.
The negative effect may occur due to: 1) competition with native pollinators, which decreases the number of flower visits by effective pollinators; 2) removal of pollen grains previously deposited on the stigma; 3) low fidelity and pollen loss due to deposition on other species; 4) differences in flower visit rates on male and female flowers, resulting in a low transferred pollen rate; 5) pollen and nectar stealing behaviour without accomplishing pollination; 6) rise of high geitonogamy
rates; and 7) deposits of heterospecific pollen, which competes on the stigma for co-specific pollen.