Universal and rapid salt-extraction of high quality genomic DNA
for PCR-based techniques.
Then proteins were eliminated by two stages of phenol/chloroform and after separation and washing, the genomic DNA
was solved in TE buffer.
The genomic DNA
was extracted from young leaves of orchid (Dendrobium crumenatum) according to the CTAB method (Pasakinskiene and Paplauskiene, 1999) with some modifications.
Two basic kinds of services are available: SigNature DNA is used to mark products, and BioMaterial Genotyping detects genomic DNA
in nature materials.
Abstract--Procedures for sampling genomic DNA
from live billfishes involve manual restraint and tissue excision that can be difficult to carry (mr and may produce stresses that affect fish survival.
was the template for most of the analyses, but additionally the cDNA synthesized for translocation detection was used as the template in case of patients with ALL.
In this second edition, Tost (epigenetics, Centre Nationale de Genotypage, CEA) and more than 70 co-contributors have thoroughly revised, updated, and expanded coverage of the most current methods known to further understand the molecular processes that identify the genomic DNA
The presence of HHV-8 in genomic DNA
was investigated in 22 of the participants at clinical onset of diabetes.
In one application, up to five loci in the genomic DNA
sequences of Helicobacter pylori were amplified.
Obtaining a sufficient amount of genomic DNA
(gDNA) while causing the least amount of harm is a critical issue.
In accordance with the manual, a whole genome amplification system (WGA; Sigma-Aldrich, Saint Louis, MO, USA), which was developed for amplification of genomic DNA
, was used to amplify viral double-stranded cDNA.
was extracted from event MON863 and analyzed using Southern blot analysis (Southern, 1975) to determine the number of transgenic insertions, the copy number of the transgenes at each insertion site, the integrity of the inserted cry3Bb1 gene, the nptII transgene cassettes, and the absence of plasmid backbone sequence.