By exploiting the fact that human and mouse genomic organization, including exon-intron boundaries, is highly conserved , the human genomic sequence was deduced by comparison of human genomic clone
AL121612 with the mouse cDNA sequence (accession number M85271).
A hammerhead ribozyme, based on the Haseloff and Gerlach model (9) was designed to cleave a pGUA sequence at nucleotide 5910 within the HIV genomic clone
NL43 (Accession number M19921) (Figure 1).
The design and cloning of an anti-tat hammerhead ribozyme targeted to one of these sites, a GUC sequence, located at nucleotide 5877 of the HIV-1 genomic clone
NL43 is described.
The primer-extended cDNA products were analyzed by electrophoresis on a 6% (w/v) urea-polyacrylamide gel in parallel with a sequencing ladder generated with the same primer and corresponding genomic clone
(2002) found a kinase sequence between two RGAs in a genomic clone
The genomic clone
, M1a, tightly linked to the Rsv3 gene, contains an LRR consensus region identical to that of the extracellular LRR class of disease resistance genes.
Genetic maps containing EST and sequenced genomic clone
markers are especially useful toward this end.
Dynamics of genomic clones
in breast cancer patient xenografts at single-cell resolution.
Rapid generation of region-specific genomic clones
by chromosome micro-dissection: isolation of DNA from a region frequently deleted in malignant melanoma.
To study the function of these structures and the regulation of precursor production we have cloned cDNA and genomic clones
encoding involucrin and four of the keratin polypeptides.
When a deletion is 100% (no copies present), a resolution of 1 to 2 Mbp can be achieved.15 The use of arrayed DNA fragments, such as large-insert genomic clones
or cDNA clones, as hybridization targets has dramatically increased the resolution of CGH,16 potentially down to ~100 kbp, and even higher resolution is possible with tiled single nucleotide polymorphism arrays.