Mutations in glucokinase
, HNF-1[alpha], HNF-1[beta], HNF-4[alpha], IPF-1, and Neuro-D1 genes linked with early onset autosomal type 2 diabetes were searched for by means of a double gradient, denaturing gradient gel electrophoresis.
GKM 001, a glucokinase
modulator, will have a clear edge over existing approaches to type 2 diabetes, believes Dr.
Among monogenic forms of T2DM, the most common is the maturity onset diabetes of the young (MODY), a genetically heterogeneous disease caused by mutations in the genes encoding hepatocyte nuclear factor-4a (MODY 1), glucokinase
(MODY 2), hepatocyte nuclear factor 1a (MODY 3), insulin promoter factor-1 (MODY 4), hepatocyte nuclear factor-1b (MODY 5) and neuro D (MODY 6).
The cytosolic glucose concentration in hepatocytes higher than most other cell types is due to the presence of a higher [K.sub.m] glucose transporter (GLUT2), a higher Km hexokinase (glucokinase
), different insulin sensitivity, and different metabolic activity [101, page 59], [102, 103].
Imachi et al., "Exendin-4 regulates glucokinase
expression by CaMKK/CaMKIV pathway in pancreatic [beta]-cell line," Diabetes, Obesity and Metabolism, vol.
"Biotin regulation of pancreatic glucokinase
and insulin in primary cultured rat islets and in biotindeficient rats." Endocrinology, 1999; 140(10):4595-600.
Our patient with hyperglycemia and dysmorphic features had a deletion of 7.23 Mb comprising the region 7p13-p12.1, with involvement of 39 Online Mendelian Inheritance in Man genes, including glucokinase
associated with maturity-onset diabetes of the young, CCM2 associated with type 2 cerebral cavernous malformations, insulin-like growth factors binding protein-3 associated with decreased postnatal growth, and oxoglutarate dehydrogenase associated with alpha-ketoglutarate dehydrogenase deficiency (short stature, hypotonia, cognitive impairment, and movement abnormalities).
sinensis had hypoglycemic effect through increasing the enzyme activities of glucokinase
, hexokinase, and glucose-6-phosphate dehydrogenase .
Kaminski et al., "T cell activation is driven by an ADP-dependent glucokinase
linking enhanced glycolysis with mitochondrial reactive oxygen species generation," Cell Reports, vol.
The supernatant was separated and divided into three parts for measurement of glucokinase
(GK), phosphoenolpyruvate carboxykinase (PEPCK), and glucose-6-phosphatase (G6Pase) activity.
Further, the molecular interaction study of the ligands 1, 2 and glibenclamide with various diabetes mellitus related protein targets like glucokinase
(PDB ID: 1V4S), fructose-1,6-bisphosphatase 1 (PDB ID: 2JJK) 11-[beta]-hydroxysteroid dehydrogenase (PDB ID: 2BEL) and modeled protein sulfonylurea receptor 1 (SUR1) showed that ligand 1 and 2 possess binding affinity with all protein targets except for 2BEL target protein for which ligand 1 has no interaction.