The relative expression of target genes was normalised with glyceraldehyde
In sugarcane, the most commonly used reference genes are: 25S ribosomal ribonucleic acid (rRNA), glyceraldehyde
3-phosphate dehydrogenase (GAPDH), [beta]-actin, [gamma]-actin, [alpha]-tubulin, [beta]-tubulin, and ubiquitin, among others.
Evaluation of a bi-epitope of glyceraldehyde
3-phosphate dehydrogenase and Disorganized Muscle in the protection of sheep against Haemonchus contortus
B, C) Viral burdens in spleen (B) and lymph node (C) after subcutaneous infection of Toro-2003 or HB171/11 ([10.sup.4] FFU, n = 5) were measured by quantitative PCR and normalized to intracellular glyceraldehyde
3-phosphate dehydrogenase (GAPDH) levels as previously described (14).
CCK-8: cell counting kit-8; ELISA: enzyme-linked immunosorbant assay; GAPDH: glyceraldehyde
3-phosphate dehydrogenase; IL: interleukin; TNF-[alpha]: tumor necrosis factor-alpha; P-: pro; C-: cleaved.
cruzi target glyceraldehyde
3-phosphate dehydrogenase (GAPDH), a glycolytic enzyme responsible for the conversion of glyceraldehyde-3-phosphate to 1, 3 -diphosphoglycerate.
phosphate dehydrogenase (GAPDH) was the reference gene for normalization.
3-phosphate dehydrogenase (GAPDH) was used as an endogenous reference gene for relative quantification.
184.108.40.206) metabolism, and responsible for the cleavage of fructose 1,6- bisphosphate in two trioses: glyceraldehyde
3-phosphate and dihydroxyacetone phosphate in glycolysis.
Besides the aforementioned virulence factors SDSE isolates have C5a peptidase, glyceraldehyde
3-phosphate dehydrogenase, and hyaluronidase .
The blots were probed with antibodies against glyceraldehyde
3-phosphate dehydrogenase (GAPDH; SAB, USA), cleaved caspase 3 (SAB, USA), cleaved poly (adenosine diphosphate-ribose) polymerase (PARP; SAB, USA), Bax (CST, USA), Bcl-2 (CST, USA), and LC 3-II and Atg7 (BIOSYNTHESIS, Beijing, China).
Further metabolic profile studies of the CRC, CD44(), and CD44(-) cells indicated that curcumin treatment increased glyceraldehyde
and hydroxypropionic acid in CD44(-) cells but decreased glutamine content in both curcumin-treated CRC and CD44() cells.