The underlying reason for severe haemolysis in G6PD deficient patient is reduced level of glutathione, which protects against free radicals that cause oxidative damage to the erythrocyte.6,7 Hence any infection to already damaged erythrocytes due to free radicals can lead to severe haemolysis, severe drop in haemoglobin and renal failure.7-9 Whereas excess haematin
and bilirubin may lead to obstruction of renal tubules, precipitating non-oliguric acute renal failure.
The faecal and blood samples were simultaneously collected from all animals of four groups to measure the magnitude of gastro-intestinal parasite using modified McMaster egg counting technique (Coles et al., 1992) and hemoglobin estimation was undertaken by using acid haematin
method using Sahli's hemocytometer (Benjamin,1985).
The slices were redyed with haematin
for 3 min and dehydrated, coverslipped, and observed under microscopy observation at the different districts (DG/CA1/CA2/CA3) of the hippocampi.
The Forrest classification classifies ulcers with a spurting haemorrhage (Forrest Ia), oozing haemorrhage (Forrest Ib), a visible vessel (Forrest IIa), an adherent clot (Forrest IIb), haematin
on the ulcer base (Forrest IIc), and a clean ulcer base (Forrest III).
The total pigments were calculated as haematin
using the following formula: total pigment (mg/kg) = A640x680.