Some erythrocytic enzymes in healthy and haemoglobinuric buffaloes.
Haemato-biochemical profile of haemoglobinuric buffaloes.
87% and 61% of all 77 haemoglobinuric animals was affected in their 3rd to 6th lactations and during winter season i.e.
In a study conducted by Samad (1997) analysis of six years haemoglobinuric buffaloes' data revealed that pregnancy and late stage of gestation were putative risk factors of parturient haemoglobinuria whereas parturition was the sparing factor.
(2006) studied post parturient haemoglobinuric
buffaloes which were treated before one year clinically and observed chronic hypophosphataemia, decreased milk yield, lameness anoestrus and change in body color ranging from brown to light grey.
Significantly (P less than 0.05) increased mean corpuscular volume (60.72+-8.49fL), mean corpuscular haemoglobin (21.46+-2.52pg), red cell distribution width (16.80+-1.81%), reticulocytes (1.25+- 0.66%), platelets (208.13+-85.26x103 /ul), plateletcrit (0.09+-0.04%), mean platelet volume (4.99+-1.85fL), serum alkaline phosphatase (164.20+-88.95u/L), bilirubin conjugated (0.40+-0.17mg/dl), bilirubin unconjugated (0.67+-0.22mg/dl) and bilirubin total (1.07+-0.39mg/dl) whereas; significantly (P less than 0.05) decreased serum calcium (2.12+-0.11mmoles/L) and phosphorous (2.67+-0.79mg/dL) were recorded in haemoglobinuric buffaloes compared to healthy controls.
These thirty haemoglobinuric buffaloes were divided into three groups for clinical trial to compare and assess the recovery rates of three different treatment packages.
The basic principle of treatment is to correct hypophosphataemia in haemoglobinuric
dairy cows and buffaloes.
A case-control epidemiological study was carried out to estimate the haematological and serum biochemical risk factors associated with parturient haemoglobinuria on 30 haemoglobinuric
and 60 apparently healthy buffaloes selected from district Chakwal during December, 2010 and January, 2011.
Therefore, it was considered appropriate to investigate the status of oxidative stress within the erythrocytes of haemoglobinuric
buffaloes during therapy with buffered inorganic phosphorus with inosine and sodium pyruvate by measuring erythrocytic MDA, GSH and erythrocytic fragility.
The colour of urine in haemoglobinuric
buffaloes ranged from red, dark red to coffee coloured depending upon the severity and duration of illness (Akhtar, 2006).
The findings with respect to haematological parameters of haemoglobinuric
animal are in agreement with Khan and Akhtar (2007); Radwan and Rateeb (2007) and Durrani et al.