For the cell count 0.1 mL of trypan blue was added to 0.9 mL of cell suspension and the cell count was conducted by a hemacytometer
. The cell viability was then checked with an optical microscope.
PMNs were counted with a hemacytometer
. Each symbol represents PMN count in a mouse, and bars correspond to median.
Lung lavage samples, collected following airway hyperresponsiveness tests or before fixing lungs for histology, were spun at 2000 rpm for 10 min, resuspended in 0.5 mL PBS, counted on hemacytometer
using trypan blue exclusion, and expressed as total cells/mouse BAL.
A cell count was performed manually using a hemacytometer
and cell concentration was adjusted to ~5x[10.sup.6] cells/ml.
Then, they were maintained at 37[degrees]C and 5% CO2 atmosphere and counted under a light microscope using the Bright-Line[TM] Hemacytometer
slide (Sigma-Aldrich Chemie GmbH, Steinheim, Germany).
melolonthae spores were counted with the aid of a hemacytometer
and optical microscope.
Sperm concentration was measured using an Improved Neubauer Chamber Hemacytometer
viewed at phase contrast (200x magnification).
Determination of cell growth: Cell density of hybridoma cells suspension was determined by trypan blue exclusion method using a hemacytometer
. Trypan blue stock solution 0.1 mL was added to 1 mL of the hybridoma cells (0.4% trypan blue solution in PBS (phosphate buffer saline), pH 7.2 to 7.3).
HDPSCs labeled with different concentrations of MIRB (12.5 [micro]g/mL-100 [micro]g/mL) were harvested and counted with a hemacytometer
. 1 x [10.sup.5] or 1 x [10.sup.6] cells from each group were transferred into 1.5-ml microcentrifuge tubes (Eppendorf, Westbury, NY, USA).
Platelets and monocytes were counted via a hemacytometer
. Monocytes were washed again, using the same buffer, and resuspended in XF assay buffer without PGE1.
The BALF was centrifuged (4[degrees]C, 800 x g, 10 min) and the sediment cells were resuspended in PBS for the total cell counts using a hemacytometer
, and cytospins were stained by a Wright-Giemsa staining kit (Jiancheng Company, Nanjing, China) for the differential cell counts .
Platelet counts of PRP and whole blood were analyzed in the hemacytometer
(XS-2100, Mindray, China).