Antigenic characterization of type B influenza viruses, by hemagglutination
total samplest (% seropositive samples, 95% CI) Post-third wave HI MN Age group, y < 5 99/160 (0.62, 93/150 (0.62, 0.54-0.69) 0.54-0.7) 5-14 155/200 (0.78, 146/199 (0.73, 0.71-0.83) 0.67-0.79) 15-24 216/320 (0.68, 188/311 (0.6, 0.62-0.73) 0.55-0.66) 25-44 187/294 (0.64, 155/283 (0.55, 0.58-0.69) 0.49-0.61) 45-64 62/138 (0.45, 52/135 (0.39, 0.36-0.54) 0.3-0.47) 65-74 38/74 (0.51, 38/74 (0.51, 0.39-0.63) 0.39-0.63) [greater than 46/71 (0.65, 39/71 (0.55, or equal 0.53-0.76) 0.43-0.67) to] 75 Region ([section]) Total 1,202 1,782 1,257 North West 561 624 337 South West 404 232 265 North East 237 526 179 East 0 292 122 Yorkshire and 0 108 354 Humber * HI, hemagglutination
inhibition assay; MN, microneutralization assay.
(*)A case is considered serologically confirmed if testing reveals an indirect fluorescent antibody (IFA) titer of [is greater than or equal to] 1:64, a complement-fixation (CF) titer of [is greater than or equal to] 1:16, or a fourfold rise in titer by the CF, IFA, microgglunatination (MA), latex agglutination (LA), or indirect hemagglutination
Samples with a hemagglutination
inhibition value [greater than or equal to]1:10 were considered positive.
As recommended by WHO, samples that were antibody-positive by MN underwent confirmatory testing by hemagglutination
inhibition assay with horse erythrocytes or by H5-specific Western blot analysis (9,10).
Virus isolation was assessed by hemagglutination
inhibition (HI) assay and neuraminidase inhibition assay by using a panel of reference serum samples (National Reference Laboratory for Avian Influenza, Harbin Veterinary Research Institute, Harbin City, China).
assay (TPHA) (Syphagen; Biokit).
assay) and nonspecific (Venereal Disease Research Laboratory [VDRL]) test results for Treponema spp.
All samples were delivered to the Kansas State Veterinary Diagnostic Laboratory for analysis by influenza matrix rRT-PCR and virus isolation on nasal swab samples and hemagglutination
inhibition (HI) assays against classical swine influenza virus (H1N1) (A/swine/Iowa/73) and the prototype swine influenza virus (H3N2) (A/swine/Texas/98) on serum samples.
inhibition antibody titers and virus neutralizing titers were highly elevated above background levels for ZIKV and yellow fever virus (YFV) compared with other viruses tested.
To detect influenza D infection, we titrated the serum samples by hemagglutination
inhibition (HI) assay using 3 antigenically distinct influenza D strains: D/swine/0klahoma/1334/2011 (D-OK lineage; D/OK) (1), D/bovine/Nebraska/9-5/2013 (D/660-lineage; D/NE) (6), and D/bovine/Yamagata/10710/2016 (D/Japan-lineage; D/Yamagata) (7).
a set of reagents for the determination of antibodies to the causative agent of syphilis in the reaction of passive hemagglutination
1 set, indicated by the participants3.