and eosin staining was performed from FFPE CNS tissues of all 4 patients and from FFPE internal organs of patients 2-4.
Rawat, from the University of Southern California in Los Angeles, and colleagues introduced a machine learning framework to identify relationships between cancer tissue morphology and hormone receptor pathway activation in breast cancer pathology samples stained with hematoxylin
and eosin (H&E).
Two of the parrots died, and histologic examination with hematoxylin
and eosin and Prussian blue stains revealed severe hepatic iron storage.
The staining methods performed for light microscopy were Hematoxylin
and Eosin (HandE) in order to study the morphology and determine the frequency of five classes of Lupus Nephritis.
Biopsies for hematoxylin
and eosin staining and direct immunofluorescence were performed.
These paraffin sections were stained with hematoxylin
The cells are uniformly round to oval with stippled chromatin and relatively abundant eosinophilic cytoplasm, consistent with a neuroendocrine adenoma (hematoxylin
and eosin, original magnification x20).
Sections were stained with hematoxylin
and eosin, and immunohistochemical staining (IHC) was performed using a biotin-peroxidase system, with labeling of the secondary antibody with a polymer (Advance HRP enzyme, Dako North America Inc., Carpinteria, CA, USA) and diaminobenzidine (DAB) as the chromogen.
Portions of liver were fixed in 10% neutral buffered formalin, embedded in paraffin, sectioned for light microscopy, and stained with hematoxylin
and eosin, Ziehl-Neelsen acid fast, and the periodic acid-Schiff (PAS) stain.
For histological analysis, paraffin sections of blastema tissues were stained with Hematoxylin&Eosin (Bio-Optica Mayer's Hematoxylin
and Eosin Y Plus) and Masson's Trichrome (Bio-Optica, 04 - 010802) according to manufacturer's protocols.
Histopathological evaluation of the gastrointestinal tract revealed loss of parietal cells, mild glandular disarray, acute inflammation, and apoptotic activity in the stomach (Figure 3, hematoxylin
and eosin, original magnification 200x) and patchy villous atrophy and absence of goblet cells and Paneth cells in the duodenum with crypt abscesses and apoptosis (Figure 3, hematoxylin
and eosin, original magnification 200x); and in the terminal ileum, as in the duodenum, there was also an absence of goblet and Paneth cells, with crypt dropout (Figure 3, hematoxylin
and eosin, original magnification 200x).
Light micrographs of rat kidney sections were stained with hematoxylin