patients who have inflammation indicated by increased C-reactive protein concentrations will usually have low haptoglobin concentrations of <30 mg/dL (2) because the haptoglobin-hemoglobin complex is cleared from the circulation with a half-life of 10 to 30 min.
Ultrasonic hemolyzation offers advantages over chemical hemolyzation, including low maintenance and zero risk of interference from chemical hemolyzing
agents or partially hemolyzed red blood cells.
Early studies of human and canine red blood cells (RBCs) showed that both could tolerate temperatures as high as 46[degrees]C for up to an hour without hemolyzing
or undergoing demonstrable morphological change (Ham, Shen, Fleming, & Castle, 1948).
2]/F controls were prediluted in 1 mL Bio-Rad hemolyzing
In this series, the viewer will learn the revised CLSI order of draw; how to avoid hemolyzing
specimens; how to minimize collector-induced errors that alter results; the most common mistakes that injure patients and bring lawsuits; and much more.
Glucose concentrations were determined in 500 [micro]L of hemolyzing
reagent plus 10 [micro]L of blood or plasma (collected in heparinized end-to-end glass capillaries; cat.
In addition to the effect hemolysis has on analytes, the presence of hemolysis may also mask hemolyzing
antibodies in the antibody screen and crossmarch.
Saponin, the traditional hemolyzing
agent, consistently inhibited total cell lysis in ascorbate at the important pH values of 4.
To maximize the release of erythrocyte folate, and thus ensure appropriate measurement of folate status, it may be necessary to use a hemolyzing
agent within the RCF assay to ensure complete release of Hb.
The procedure begins with an autodiluter aspirating 5 [micro]L of blood and dispensing it with 1 mL of hemolyzing
CO-Oximetry interference by perflubron emulsion: comparison of hemolyzing
and nonhemolyzing instruments.
After the patients' blood samples were mixed manually with the hemolyzing
and other reagents for the IMx glycohemoglobin assay in the same proportions as are used in this analyzer, numerous intact leukocytes (lymphocytes) could be observed by microscopy.