Microsatellite data analysis: Popgene 1.32 (Yeh and Boyle 1997) was used to estimate the number of alleles (NA) and effective number of alleles (NE), and CERVUS 3.0 (Kalinowski et al., 2007) was employed to calculate the polymorphic information content (PIC), expected heterozygosity
(HE) and observed heterozygosity
These features include the analysis of human leukocyte antigen loss of heterozygosity
, the presence (or absence) of seven of the most common oncogenic viruses, as well as the composition of the T-cell receptor alpha repertoire, which is complementary to the previously-released TCR beta analysis.
Statistical analysis for calculation of genetic variability measures such as number of alleles, expected and observed heterozygosity
, homozygosity and Polymorphic Information Content (PIC) was calculated for all microsatellite markers.
The number of alleles (NA), observed (HO) heterozygosity
and expected (HE) heterozygosity
were calculated using ARLEQUIN ver.
The converted data was processed using POPGEN version 1:32 program  to generate the observed number of alleles ([n.sub.a]), effective number of alleles ([n.sub.e]), observed heterozygosity
value ([H.sub.o]), expected heterozygosity
value ([H.sub.e]), genetic differentiation ([F.sub.ST]), the global heterozygote deficit among breeds ([F.sub.IT]), and the heterozygote deficit within the breed ([F.sub.IS]), gene flow (Nm), Hardy-Weinberg equilibrium (HW), and allele frequency.
The number of allele, and expected and observed heterozygosity
were characterized in the wild population of Panjin, China.
The three DNA abnormalities examined in the study, all of which are included in the PancraGEN(R) assay, included: elevated DNA quantity, KRAS mutation, and loss of heterozygosity
mutations among a panel of key tumor suppressor genes.
Arlequin software version 3.5 was used to calculate allele frequencies and to obtain expected heterozygosity
(He), observed heterozygosity
(Ho) and probability value (p-value) of Hardy-Weinberg equilibrium (7).
In this present study, the cultured population showed a higher level of inherent genetic diversity and allele richness than the wild as revealed by indicators such as the percentage of polymorphic loci (%P), Number of Alleles (Na), Number of effective alleles (Ne), Shannon Information index (I), and expected heterozygosity
(Nei's gene diversity).
According to Hardy-Weinberg, and to the fixation index (f), the intrapopulation genetic variability has been quantified through the number of alleles per locus (A), the percentage of polymorphic loci (P) and the observed (Ho) and expected (He) heterozygosity
(Ho) (Hamrick, 1983).
Different measurements of within breed genetic variations like observed and expected heterozygosity
, Shannon index and polymorphic parameters (the number of actual alleles and the number of effective alleles) were calculated using POPGENE software package (Yeh et al., 1999).