horseradish peroxidase


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Related to horseradish peroxidase: alkaline phosphatase
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Noun1.horseradish peroxidase - an enzyme used in immunohistochemistry to label antigens and their antibodies
peroxidase - any of a group of enzymes (occurring especially in plant cells) that catalyze the oxidation of a compound by a peroxide
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References in periodicals archive ?
Nonimmunologic binding of horseradish peroxidase to hepatitis B surface antigen.
The amount of RCA products is measured by hybridization with DNA probes conjugated to horseradish peroxidase, acting on the substrate 3,3`,5,5`,-tetramethylbenzidine to generate colorimetric reaction products that are read out with an absorbance microplate reader (Fig.
Dunford, "Horseradish peroxidase: structure and kinetic properties," in Peroxidases in Chemistry and Biology, J.
Toxicity of textile dyes and their degradation by the enzyme horseradish peroxidase (HRP).
Then strips were incubated with goat anti-mouse IgG Fc specific conjugated to horseradish peroxidase (HP) (1:5000) for 1 h at room temperature followed by vigorous washing with TBST and addition of chemi-luminescent substrate (Vector Lab) for HP.
"We showed that it's much less sensitive to pH and temperature than the natural enzyme horseradish peroxidase, a traditional means of enhancing sensitivity."
[29] reported the best decolourization of Direct Yellow 12 using horseradish peroxidase at pH of 4.0.
Horseradish peroxidase (HRP), an enzyme which catalyzes the oxidation of a wide variety of organic and inorganic substrates in the presence of [H.sub.2][O.sub.2], was also used [18].
Horseradish peroxidase, glucose oxidase from Aspergillus niger, and tetramethylbenzidine (TMB) were bought from Sigma Aldrich, Germany.
Class I is composed of intracellular peroxidases such as yeast cytochrome c peroxidase, ascorbate peroxidase, and catalase peroxidase, Class II is formed by secretory fungal peroxidases such as manganese and lignin peroxidases, and Class III consists in secretory plant peroxidases including one of the most studied peroxidases, the horseradish peroxidase [4,5].