horseradish peroxidase


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Related to horseradish peroxidase: alkaline phosphatase
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Noun1.horseradish peroxidase - an enzyme used in immunohistochemistry to label antigens and their antibodies
peroxidase - any of a group of enzymes (occurring especially in plant cells) that catalyze the oxidation of a compound by a peroxide
References in periodicals archive ?
2009), horseradish peroxidase for detoxification of wastewater (Caramori, Fernandes, & Carvalho Junior, 2012) and F1 antigen from Yersinia pestis (Barbosa et al.
Reagent and procedure used in these procedures using the Horseradish peroxidase (HRP), the Peroxidase-anti-peroxidase (PAP), the Avidin-biotin complex (ABC), or the Alkaline phosphatase-anti-alkaline-phosphatase (APAAP) for detection of antigen-specific antibodies in frozen or paraffin sections are necessary for the validation of the staining results.
Then strips were incubated with goat anti-mouse IgG Fc specific conjugated to horseradish peroxidase (HP) (1:5000) for 1 h at room temperature followed by vigorous washing with TBST and addition of chemi-luminescent substrate (Vector Lab) for HP.
The enzyme-free detection system is based on the electrochemical properties of Fe 3O4 nanoparticles, which have been known to mimic the peroxide-reducing capacity of horseradish peroxidase.
After washing, horseradish peroxidase (HRP) conjugated anti- human IgG immunologically detects the bound patient antibodies forming a conjugate-antibody- antigen complex.
Horseradish peroxidase Type II (Sigma) was used as enzyme standard.
The following materials were obtained commercially and used as received: horseradish peroxidase (HRP) (500 U/mg, MW40 000, SERVA), H2O2 (analytical grade, Shanghai Chemical Plant, China), ionic liquid 1-ethyl-3-methylimidazolium hexafluorophosphate (EMIMPF6, 97%, melting point 58 ~ 62 oC, Guangzhou Weibo Chemical Limited Company, China), and m-aminophenol (MAP) (Beijing Hengyezhongyuan Limited Company, China).
Rocha et al (45) used peroxidase of bovine erythrocytes of 680 units/mg of proteins at a concentration of 10 mg/ml, while for the present study we applied horseradish peroxidase of 150units/mg of proteins at a concentration of 472 mg/ml, increasing the final quantity of weight/volume of the enzyme, with better results in terms of recovery of bond strength to dental enamel following whitening.
Some low molecular weight enzymes such as horseradish peroxidase (HRP), cytocrome c peroxidase and fungal peroxidase, commonly used for building amperometric biosensors, present a kinetic barrier for the direct electron transfer between the active sites of redox enzymes and the electrode surface (KUBOTA et al.
Horseradish peroxidase (HRP) conjugated anti-human IgG immunologically detects the bound patient antibodies forming a conjugate / antibody / antigen complex.