2009), horseradish peroxidase
for detoxification of wastewater (Caramori, Fernandes, & Carvalho Junior, 2012) and F1 antigen from Yersinia pestis (Barbosa et al.
infantum and the reporter horseradish peroxidase
catalyzed degradation of industrially important dyes.
Reagent and procedure used in these procedures using the Horseradish peroxidase
(HRP), the Peroxidase-anti-peroxidase (PAP), the Avidin-biotin complex (ABC), or the Alkaline phosphatase-anti-alkaline-phosphatase (APAAP) for detection of antigen-specific antibodies in frozen or paraffin sections are necessary for the validation of the staining results.
Then strips were incubated with goat anti-mouse IgG Fc specific conjugated to horseradish peroxidase
(HP) (1:5000) for 1 h at room temperature followed by vigorous washing with TBST and addition of chemi-luminescent substrate (Vector Lab) for HP.
The enzyme-free detection system is based on the electrochemical properties of Fe 3O4 nanoparticles, which have been known to mimic the peroxide-reducing capacity of horseradish peroxidase
After washing, horseradish peroxidase
(HRP) conjugated anti- human IgG immunologically detects the bound patient antibodies forming a conjugate-antibody- antigen complex.
Type II (Sigma) was used as enzyme standard.
The following materials were obtained commercially and used as received: horseradish peroxidase
(HRP) (500 U/mg, MW40 000, SERVA), H2O2 (analytical grade, Shanghai Chemical Plant, China), ionic liquid 1-ethyl-3-methylimidazolium hexafluorophosphate (EMIMPF6, 97%, melting point 58 ~ 62 oC, Guangzhou Weibo Chemical Limited Company, China), and m-aminophenol (MAP) (Beijing Hengyezhongyuan Limited Company, China).
Rocha et al (45) used peroxidase of bovine erythrocytes of 680 units/mg of proteins at a concentration of 10 mg/ml, while for the present study we applied horseradish peroxidase
of 150units/mg of proteins at a concentration of 472 mg/ml, increasing the final quantity of weight/volume of the enzyme, with better results in terms of recovery of bond strength to dental enamel following whitening.
Some low molecular weight enzymes such as horseradish peroxidase
(HRP), cytocrome c peroxidase and fungal peroxidase, commonly used for building amperometric biosensors, present a kinetic barrier for the direct electron transfer between the active sites of redox enzymes and the electrode surface (KUBOTA et al.
(HRP) conjugated anti-human IgG immunologically detects the bound patient antibodies forming a conjugate / antibody / antigen complex.