hybridoma


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hy·brid·o·ma

 (hī′brĭ-dō′mə)
n.
A cell that is produced in the laboratory by fusing an antibody-producing lymphocyte (which does not readily divide) with a nonantibody-producing cancer cell (which divides rapidly). The hybridoma proliferates and produces a continuous supply of a specific monoclonal antibody.

hybridoma

(ˌhaɪbrəˈdəʊmə)
n
(Biochemistry) a hybrid cell formed by the fusion of two different types of cell, esp one capable of producing antibodies, but of limited lifespan, fused with an immortal tumour cell
[C20: from hybrid + -oma]

hy•brid•o•ma

(ˌhaɪ brɪˈdoʊ mə)

n., pl. -mas.
a hybrid cell made in the laboratory by fusing a normal cell with a cancer cell, usu. a myeloma or lymphoma, in order to combine desired features of each, as the ability of the cancer cell to multiply rapidly with the ability of the normal cell to dictate the production of a specific antibody.
[1975–80]
ThesaurusAntonymsRelated WordsSynonymsLegend:
Noun1.hybridoma - a hybrid cell resulting from the fusion of a lymphocyte and a tumor cell; used to culture a specific monoclonal antibody
somatic cell, vegetative cell - any of the cells of a plant or animal except the reproductive cells; a cell that does not participate in the production of gametes; "somatic cells are produced from preexisting cells";
Translations
hybridome

hy·brid·o·ma

n. hibridoma, célula somática híbrida capaz de producir anticuerpos.
References in periodicals archive ?
Service Providers offer support for different types and steps of antibody production?from peptide design, synthesis, and carrier protein conjugation to animal immunization, serum collection, or hybridoma fusion, titer analysis, and final antibody purification.
Distributed Bio's computational immunology expertise also enables optimisation of existing client antibodies generated through more traditional platforms, such as hybridoma or traditional phage display.
Our service portfolio includes mouse and rat monoclonal antibody production using hybridoma technology, human, monkey, rabbit, chicken, dog, llama, and camel monoclonal antibody production using various antibody library technologies (including phage display, bacterial display and yeast display).
Production of mAbs was first introduced in 1975 using cell fusion technique and hybridoma cell production.
The hybridoma cells producing antibodies against rAra h 2 were screened by indirect ELISA and subcloned by limiting dilution to obtain stable mAb-producing cell lines.
To test the specificity of the newly established mAb K81116-6, the antibodies were removed from the hybridoma supernatant (depletion) and the supernatant was then used for immunofluorescence analysis.
HEK293T cell line, SP2/0 mouse myeloma cells, and monoclonal anti-Plexin C1 antibody-secreting hybridoma cells were cultured in high glucose DMEM supplemented with 10% FBS and antibiotics.
It allows IPA to become a single source provider of services across the full antibody discovery value chain (antigen design, hit generation, lead selection, lead optimisation and lead characterisation) and to offer antibody production methodologies (library based technologies, hybridoma methods, transgenic animal based platforms and single B cell based technology).
For the preparation of hybridoma conditioned medium (feeder), mouse macrophages of the BALB/c strain were obtained by the intraperitoneal lavage procedure.
[13] Other study also demonstrate that G-CSF, augment ChAT activity in primary cultured neurons and in cholinergic hybridoma cell line.
In the present study, hybridoma technology has been used to develop a murine hybridoma cells producing monoclonal antibody by the conditioning of cell culture media with different carbohydrates in vitro.
mAbs 3A8.1 ([IgG.sub.1]k) and 2B9.3 ([IgG.sub.2a]k) were obtained from fusions 4399 and 4403, respectively at the Washington University Hybridoma Facility following a DNA vaccine/ recombinant protein immunization schedule (4).