After treating with the corresponding biotin antibody, HRP Avidin, and TMB substrate, these 2-fold dilution series were adopted to determine the optical density of each well within 5 minutes under 450 nm using an automatic enzyme-linked immunoadsorbent
Louis, MO) was dissolved in PBS and incubated for 1 hour at 37[degrees]C with immunoadsorbent
PAD2 and PAD4 enzymes.
CSE, TLR4, IL-10, IL-12, and TNF-[alpha] levels in the renal homogenates were determined using a rat enzyme-linked immunoadsorbent
assay (ELISA) kit and commercially available antibodies (R&D Systems, USA).
The fever outbreak was declared as dengue outbreak by the District health authorities and confirmed through laboratory confirmatory tests (Using Standardized dengue Enzyme-linked immunoadsorbent
assay for immunoglobulin M).
Supernatants were collected after 18 to 22 hours and IgG and IgM concentrations were determined by standard enzyme-linked immunoadsorbent
assay (ELISA), as previously described .
Phenylalanine modified CaP (CaP-PA) was studied as a control since phenylalanine modified matrices are commercially used in immunoadsorbent
To remove the nonspecific antibodies, an immunoadsorbent
was prepared by polymerizing sexually undifferentiated oyster tissue using glutaric dialdehyde according to Fuchs and Sela (1973).
We determined in all samples: baseline plasminogen activator inhibitor (PAI-1) activity by enzyme- linked immunoadsorbent
assay (ELISA-Innogenetics NY, Antewerp, Belgium).
Plasma TNF-[alpha] concentrations were determined by enzyme-linked immunoadsorbent
assays using commercially available kits (R&D Systems, Minneapolis, MN).
Her enzyme-linked immunoadsorbent
assay is positive for HIV antibodies, and her HIV infection is confirmed with a Western blot test.
This test is an enzyme-linked immunoadsorbent
assay for bacterial protein.
Stool samples from 76 consecutive, hospitalized patients were analyzed utilizing routine bacterial cultures, smears for identification of ova and parasites and Enzyme-Link Immunoadsorbent
Assay (ELISA) for enteric bacteria, parasites and viruses.