immunoblot


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Related to immunoblot: immunoprecipitation

im·mu·no·blot

 (ĭm′yə-nō-blŏt′, ĭ-myo͞o′-)
n.
A laboratory procedure, such as Western blot analysis, in which proteins that have been separated by electrophoresis are transferred to a membrane of nitrocellulose or another material and are identified by their reaction with labeled antibodies.
intr. & tr.v. im·mu·no·blot·ted, im·mu·no·blot·ting, im·mu·no·blots
To perform or cause to be subjected to an immunoblot.

immunoblot

(ˌɪmjʊnəʊˈblɒt)
n
a technique used to transfer a pattern of proteins
Translations
inmunotransferencia

Western Blot

, immunoblot
n. Western Blot, “inmunoblo'', prueba subsecuente para confirmar la infección por el virus VIH, en pacientes con evidencia de exposición, indicada por un ensayo enzimático inmuno-sorbente (ELISA).
References in periodicals archive ?
Of this group, 477 participants underwent further follow-up testing using a recombinant immunoblot assay.
Hence, it was determined to compare Widal test with Immunoblot test results for accurate and early diagnosis of typhoid fever.
Molecular tests of blood and skin biopsy and serologic testing for Borrelia burgdorferi sensu lato and syphilis were repeatedly negative, except for a C6 EIA IgM/IgG seroconversion (Immunetics, Boston, MA, USA) in convalescent-phase serum samples that was positive but could not be confirmed by either IgM or IgG immunoblot (Mikrogen, Neuried, Germany) (online Technical Appendix Table 2).
Serial unstained sections were cut from these FFPE and frozen blocks and stained for CD155 by IHC or immunoblot. Raji cells (Cat.
Ubiquitination was detected by immunoblot using Flag antibody (Sigma), and CIITA ubiquitination was verified with GST (Abcam), and pCAF was verified with monoclonal a pCAF antibody (Santa Cruz).
Immunoblot analysis revealed that IL-1[beta] maturation occurred in response to [DELTA]nleF, but not EDL933 (or [DELTA]nleF/nleF) infection, suggesting that [DELTA]nleF infection induced pyroptosis-like cell death.
Anti-smooth muscle antibodies, Anti-mitochondrial antibodies and Anti-liver kidney muscle (LKM) antibodies were negative by Western immunoblot. Magnetic resonance cholangio-pancreatography (MRCP) was inconclusive owing to compressed Intra-hepatic biliary channels (figure 1).
An immunoblot was performed to examine whether the HO-1 that was induced by [beta]-glucan would control the expressions of the NO and the HO-1 that were mediated by E.
Methods: We evaluated cell survival by MTT assay, apoptosis and DNA content by flow cytometry, protein expression and phosphorylation by immunoblot analyses.
The immunoblot should not be run without first performing an EIA or IFA.
Com proteins were quantified; immunoblot analysis determined the abundance.