immunoblotting


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Related to immunoblotting: immunoprecipitation

im·mu·no·blot

 (ĭm′yə-nō-blŏt′, ĭ-myo͞o′-)
n.
A laboratory procedure, such as Western blot analysis, in which proteins that have been separated by electrophoresis are transferred to a membrane of nitrocellulose or another material and are identified by their reaction with labeled antibodies.
intr. & tr.v. im·mu·no·blot·ted, im·mu·no·blot·ting, im·mu·no·blots
To perform or cause to be subjected to an immunoblot.
American Heritage® Dictionary of the English Language, Fifth Edition. Copyright © 2016 by Houghton Mifflin Harcourt Publishing Company. Published by Houghton Mifflin Harcourt Publishing Company. All rights reserved.

immunoblotting

(ˌɪmjʊnəʊˈblɒtɪŋ)
n
the act or process of making immunoblots
Collins English Dictionary – Complete and Unabridged, 12th Edition 2014 © HarperCollins Publishers 1991, 1994, 1998, 2000, 2003, 2006, 2007, 2009, 2011, 2014
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Fractions were collected from each peak, after measuring the concentrations of their protein, these fractions were performed to SDS-PAGE and immunoblotting for detecting the allergenic activity.
HCT-8 cells were stably transfected with human OAZ2 cDNA clone, and OAZ2 overexpression was verified by immunoblotting (Figure 5(a)).
Then, the beads were washed four times with lysis buffer, and the bound proteins were analyzed by immunoblotting using the indicated antibodies.
Anti-Smith (Sm) antibody, which was first described by Tan and Kunkel,[sup][1] is specific for adult systemic lupus erythematosus (SLE) according to the American College of Rheumatology.[sup][2] However, the frequency of anti-Sm antibody in adult SLE patients is low (ranging from 5% to 30%) depending on the serologic tests used and the ethnic origin of the patient population.[sup][3],[4] More researchers have exploited various techniques, such as immunoblotting, immunofluorescence, and enzyme-linked immunosorbent assay (ELISA), to increase anti-Sm antibody detection sensitivity.[sup][3],[5],[6],[7],[8]
To identify the allergen(s) in the cyanobacteria that is binding to IgE, we performed 2D gel electrophoresis, followed by immunoblotting, using MC(-) lysate and pooled patient sera.
Immunoblotting findings were largely divided into three patterns (Figure 1): pattern 1: nonglycosylated form (NG) dominant (corresponding to percentage of glycosylation <40% in Figure 3), pattern 3: glycosylated form (G) dominant (corresponding to percentage of glycosylation >60% in Figure 3), and pattern 2: intermediate between patterns 1 and 3.
Immunoblotting. The WCA and SSA of all the isolates separated on 12.5% (w/v) SDS-PAGE slabs [21] were transferred electrophoretically on nitrocellulose membrane papers (NCP) (Sartorius).
The results of immunoblotting inhibition revealed that the IgE binding reactivity of the allergenic proteins with 12, 20, 39, and 45 from the A.
Immunoblotting revealed that DAT substrates led to increased DAT T53 phosphorylation.
To collect urine, both ureters were exposed and catheterized through a midline incision as described by Topcu and colleagues.[sup.15] Before sacrificing the animals, we drew about 2 mL of artery blood from the abdominal aorta in gas-tight syringes; both kidneys were then quickly removed for immunoblotting. The blood was centrifuged for 15 minutes at 4000 g to remove the blood cells, and the plasma was subsequently analyzed for sodium, potassium, creatinine and urea (Hitachi 7600-020 automatic biochemical analyzer, Hitachi High-Technologies, Tokyo, Japan).