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Related to immunofluorescence microscopy: Confocal microscopy


 (ĭm′yə-nō-flo͝o-rĕs′əns, -flô-, -flō-)
Any of various techniques that use antibodies chemically linked to a fluorescent dye to identify or quantify antigens in a tissue sample.

im′mu·no·fluo·res′cent adj.


(ˌɪmjʊnəʊflʊəˈrɛsəns) or


(Medicine) a method used to determine the location of antibodies or antigens in which the antibodies or antigens are labelled with a fluorescent dye


(ˌɪm yə noʊ flʊˈrɛs əns, -flɔ-, -floʊ-, ɪˌmyu-)

any of various techniques for detecting an antigen or antibody in a sample by coupling its specifically interactive antibody or antigen to a fluorescent compound, mixing with the sample, and observing the reaction under an ultraviolet-light microscope.
ThesaurusAntonymsRelated WordsSynonymsLegend:
Noun1.immunofluorescence - (immunology) a technique that uses antibodies linked to a fluorescent dye in order to study antigens in a sample of tissue
technique - a practical method or art applied to some particular task
immunology - the branch of medical science that studies the body's immune system


n. inmunofluorescencia, método que usa anticuerpos marcados con fluorescina para localizar antígenos en los tejidos.
References in periodicals archive ?
We thank all hunters for their help during fieldwork, Joerg Roesner for help with immunofluorescence microscopy, and Sarah Schulz for providing HEV antibody reference material.
Additional tissue fragments are required to carry out electron and immunofluorescence microscopy analyses to enable a definitive diagnosis (CIANCIOLO et al., 2013).
Caption: FIGURE 2: Immunofluorescence microscopy findings.
Caption: Figure 2: Immunofluorescence microscopy. Granular predominantly mesangial staining for the immunoglobulins (a) IgG (1+), (b) IgM (1+), and (c) IgA (1+) and complement components (d) C3 (2+) and (e) C1q (1+).
The present knowledge of the pathology of renal diseases has been derived to a large extent after the introduction of percutaneous needle biopsy of the kidney and the systematic study of these small samples of renal tissue by light microscopy, electron microscopy and immunofluorescence microscopy. The primary role of the renal biopsy is to provide a diagnosis that allows the clinician to make an informed prognosis and assign lesion-specific therapy.
Immunofluorescence microscopy testing (for IgG, IgA, IgM, C3, C1q, ?, and e) indicated completely negative results.
They are both blistering diseases and on immunofluorescence microscopy show evidence of IgA deposition; however, dermatitis herpetiformis is associated with a gluten-sensitive enteropathy (coeliac disease).
Diagnosis can be made with a proper clinical history and examination, tissue pathology, direct immunofluorescence microscopy (DIF), and / or serology, with DIF being the most definitive.
The HEp-2000 substrate is similar to the standard HEp-2 substrate but has Sjogren's syndrome A (Ro/SSA) complementary DNA transfected into it to create a unique 'SSA/speckled' pattern on immunofluorescence microscopy. Anti-ENA testing was performed by enzyme-linked immunosorbent assay (Immuno Concepts) and anti-dsDNA testing via EIA (Immuno Concepts) and Farr radioimmunoassay (Siemens Healthcare Diagnostics, Erlangen, Germany).
Immunofluorescence microscopy showed no staining for IgG, IgA, IgM, fibrinogen, C1q, and C3 (Figure 1, 2).
Methods: Cell fractionation, western blotting, and immunofluorescence microscopy experiments were performed to measure [beta]-catenin protein levels and [beta]-catenin cellular localization in HEK293Tcells transfected with various amounts of a [beta]-catenin-encoding plasmid.