Cells were lysed with
immunoprecipitation assay lysis buffer (RIPA, Sigma).
A simplified
immunoprecipitation method for quantitatively measuring antibody responses in clinical sera samples by using mammalian-produced Renilla luciferase-antigen fusion proteins.
The isolated Nuclear/ Cytoplasmic proteins were subjected to
immunoprecipitation and Western-blot experiments.
Next, we performed chromatin
immunoprecipitation analysis to examine in vivo association of LexA-Med6p derivatives, Med14 (for Mediator), and TBP (for transcription initiation) with a target promoter region ([8xlexA.sub.op]).
The activation of IRS4 by IGF-1 was assessed by
immunoprecipitation of IRS-4 followed by immunoblotting with anti-PY99 antibody.
(c) The film images showed the representative blots for AR and VAPA in membrane and cytoplasmic fractions, before (input) or after
immunoprecipitation (co-IP) by the anti-AR antibody in TM4 cells treated with 10 nM testosterone for 30 min.
To verify our hypothesis, we performed an endogenous
immunoprecipitation assay.
Chromatin
Immunoprecipitation. Cultured HCT116 cells were washed and cross-linked using 1% formaldehyde for 20 min, and mouse colon tissues were cross-linked using 1% formaldehyde for 30 min and homogenized.
Anti-MDA5 autoantibody levels were measured by qualitative
immunoprecipitation assay.
Highly sensitive
immunoprecipitation method for extracting and concentrating low-abundance proteins from human serum.
The
immunoprecipitation analysis confirmed that, Oak1 protein was produced in all the tested transformants.