The
immunoprecipitation analysis confirmed that, Oak1 protein was produced in all the tested transformants.
The following primary antibodies were used: NF-[kappa]B p65 (chromatin
immunoprecipitation (ChIP) grade, #ab7970) was obtained from Abeam (Cambridge, MA, USA).
Following
immunoprecipitation with anti-ANT antibody, we analyzed the presence of ANT and Cyp-D by Western blotting with specific antibodies.
The
Immunoprecipitation assays revealed that the mutated TP53 did not bind to RAD51 in comparison to WT TP53.
Four mass spectra (represented in red, blue, green and orange) were obtained from one
immunoprecipitation.
Furthermore, blockading potential PGI(2) receptors, including
immunoprecipitation (IP) receptors and peroxisome proliferator-activated receptors alpha (PPAR alpha) and delta, revealed that siRNA-mediated blockage of PPAR alpha considerably reduced the anti-proliferation effect of L-carnitine.
The authors used the luciferase reporter assay and Western blot analysis to determine COX-2 induction by Cr(VI); dominant negative mutant, genetic knockout, gene knockdown, and chromatin
immunoprecipitation approaches were used to elucidate the signaling pathway leading to COX-2 induction.
Recent observations from some members of the WG have demonstrated degradation of the NIST SRM 2921 cTnI material via the use of
immunoprecipitation coupled with fluorescence Western blot analysis with codifferential anti-cTnI monoclonal antibodies (12).
Using a new technique known as chromatin
immunoprecipitation assay, the investigators were also able to show changes in proteins (known as histones) that can activate the gene that produces MCP-1.
of Oslo, Norway) introduces chromatin
immunoprecipitation (ChIP) as a powerful technique whereby a protein of interest is selectively immunoprecipated from a chromatin preparation to determine the DNA sequences associated with it in regulating gene expression.
Both of these new Histone antibodies have been tested for use in chromatin
immunoprecipitation (ChIP), Western blotting (WB) and Enzyme-linked immunosorbent assay (ELISA).
Chromatin
immunoprecipitation assay (CHIP) showed a histone H3-free region at the extreme 5' end of the MLL bcr suggesting this is a nucleosome-depleted region.
