Cells were lysed with immunoprecipitation
assay lysis buffer (RIPA, Sigma).
A simplified immunoprecipitation
method for quantitatively measuring antibody responses in clinical sera samples by using mammalian-produced Renilla luciferase-antigen fusion proteins.
The isolated Nuclear/ Cytoplasmic proteins were subjected to immunoprecipitation
and Western-blot experiments.
Next, we performed chromatin immunoprecipitation
analysis to examine in vivo association of LexA-Med6p derivatives, Med14 (for Mediator), and TBP (for transcription initiation) with a target promoter region ([8xlexA.sub.op]).
The activation of IRS4 by IGF-1 was assessed by immunoprecipitation
of IRS-4 followed by immunoblotting with anti-PY99 antibody.
(c) The film images showed the representative blots for AR and VAPA in membrane and cytoplasmic fractions, before (input) or after immunoprecipitation
(co-IP) by the anti-AR antibody in TM4 cells treated with 10 nM testosterone for 30 min.
To verify our hypothesis, we performed an endogenous immunoprecipitation
. Cultured HCT116 cells were washed and cross-linked using 1% formaldehyde for 20 min, and mouse colon tissues were cross-linked using 1% formaldehyde for 30 min and homogenized.
Anti-MDA5 autoantibody levels were measured by qualitative immunoprecipitation
Highly sensitive immunoprecipitation
method for extracting and concentrating low-abundance proteins from human serum.
analysis confirmed that, Oak1 protein was produced in all the tested transformants.