(%) of second specimens 112 (67) 2,942 (17.7) Interval from first to second specimen collection (days) 0-13 4 (3.6) 486 (16.5) 14-28 104 (92.9) 520 (17.7) [greater than or equal to] 29 3 (2.7) 782 (26.6) Unknown/Not reported 1 (0.9) 1,154 (39.2) Median (IQR) 19 (16-23) 24 (13-47) Test characteristics IFA IgG performed 107 (95.5) 1,618 (55.0) IFA IgG titer distribution ([section]) <1:64 3 (2.8) 67 (4.1) [greater than or equal to] 1:64 104 (97.2) 1,549 (95.7) [greater than or equal to] 1:128 92 (90.0) 957 (59.1) Abbreviations: IFA = indirect immunofluorescence
assay; IgG=immunoglobulin G; IQR = interquartile range.
The new Iterative Indirect Immunofluorescence
Imaging (4i) analysis method can also be used to determine the effects of pharmacological substances on the organization and physiology of cells.
Assay (IFA): The IFA was carried out at room temperature in a moist chamber.
Evaluation of antinuclear antibodies by indirect immunofluorescence
and line immunoassay methods: four years' data from Turkey.
Visceral Leishmaniasis confirmation criteria included: (1) visualization of amastigotes in bone marrow aspirate or other clinical materials (except when detected exclusively on skin or nasal/oral mucous membranes); (2) serology (detection of anti-rK39 antibodies by immunochromatography or indirect immunofluorescence
reaction in a title equal or greater than 1:80), associated with clinical and laboratory findings compatible with the disease.
Caption: Figure 8: The indirect immunofluorescence
test on the flask propagated UPM0081BGMP15 and UPM190BGMP15 showing low amount of IBDV antigen within the cytoplasm of infected BGM-70 cells at 72 hours after inoculation.
. Six out of 354 (1.8%) serum samples were positive for antibody to R.
Roh et al., "Evaluation of an automated screening assay, compared to indirect immunofluorescence
, an extractable nuclear antigen assay, and a line immunoassay in a large cohort of Asian patients with antinuclear antibody-associated rheumatoid diseases: A multicenter," Journal of Immunology Research, vol.
Assay Clinical SELENA-SLEDAI QF dsDNA 1.08 [+ or -] 0.24 p <0.001 ([R.sup.2] = 0.149) HA anti-dsDNA 0.77 [+ or -] 0.28 p = 0.006 ([R.sup.2] = 0.065) QL anti-dsDNA 0.69 [+ or -] 0.4 p = 0.094 ([R.sup.2] = 0.023) CLIFT anti-dsDNA 0.49 [+ or -] 0.3 p = 0.21 ([R.sup.2] = 0.008) PGA QF dsDNA 0.08 [+ or -] 0.03 p = 0.008 ([R.sup.2] = 0.053) HA anti-dsDNA 0.03 [+ or -] 0.03 p = 0.17 ([R.sup.2] = 0.007) QL anti-dsDNA -0.05 [+ or -] 0.05 p = 0.34 ([R.sup.2] = 0005) CLIFT anti-dsDNA 0.07 [+ or -] 0.05 p = 017 ([R.sup.2] = 0010) QF: QUANTA Flash; HA: high affinity; QL: QUANTA Lite; CLIFT Crithidia luciliae indirect immunofluorescence
Slides with paraffin sections of the frontal lobe of the fetal brain and the unaffected age-matched control were stained with recombinant monoclonal antibody to Flavivirus group antigen (D1-4G2-4-15 [4G2]) from Absolute Antibody (Oxford, England) and maternal sera containing immunoglobulin G antibodies against Zika virus, using the indirect immunofluorescence
technique to determine viral localization.
Caption: This photomicrograph shows respiratory syncytial virus (RSV) using an indirect immunofluorescence
For indirect immunofluorescence
analyses BG01V/hOG cells were seeded in 48-well plates (Corning Life Sciences, http://www.corning.com) at 21,000 cells/[cm.sup.2].