method was used for the determination of urease activity by measuring ammonia production as defined by Weatherburn .
Ammonia determinations based on indophenol
formation with sodium salicylate.
N using the colorimetric method indophenol
blue reaction (Strickland & Parsons 1972, Parsons et al.
Total ammonia was calculated by indophenol
blue method and alkalinity by the neutralization titrating method at the start and finish of the experiment.
2]; Winkler method with azide modification) and total ammonia nitrogen (TAN; indophenol
The rumen fluid ammonia (NH3-N) concentrations was measured using spectrophotometer (Perkin Elmer, Waltham, MA, USA) after sample prepared by ammonia reaction with alkaline hypochlorite and phenol in the presence of a catalyst (sodium nitroprusside) to form indophenol
(blue; Berthelot reaction).
3], concentrations were determined using a 2 M KCl extraction method (Keeney and Nelson 1982; Rayment and Lyons 2011) followed by colourimetric analysis at 630 nm using an indophenol
blue reaction on a Seal AA1 segmented flow analyser (Fluidquip, Sydney, NSW, Australia) (Searle 1984; Rayment and Lyons 2011).
2]; Winkler method (with azide modification) and total ammonia nitrogen (TAN; indophenol
method) were determined weekly (0800-0900).
0]Brix was determined by using hand refractometer and was expressed in percent, titratable acidity was determined as citric acid by titrating against N/10 NaOH alkali solution and was expressed in percent, ascorbic acid or Vitamin-C present in fruit was determined by using dye 2,6 dichlorophenol indophenol
(DCPIP) through visual titration method and was expressed in g per 100g of pulp whereas reducing, non-reducing and total sugar were estimated by Lane and Eynon's method and were expressed in percent (AOAC, 2000).
(DCPIP) indicator (Hanson, Desai, & Desai, 1997).
Ascorbic acid was determined by indophenol
dye method as stated in AOAC (2000).
The RAN content in the ruminal fluid samples was evaluated using an indophenol
colorimetric method (method INCT-CA N-006/1; Detmann et al.