Also, neuroendocrine tumors would be typically negative for cytokeratin 7, inhibin
, and calponin.
Serum levels of follicle-stimulating hormone, oestradiol and inhibin
B to measure ovarian reserve have the limitation of being cycle dependent.
To increase fecundity in sheep, many strategies have been followed such as genetic methods including selection of breeding programs, and non-genetic methods including administration of exogenous gonadotropins such as equine chorionic gonadotropin (eCG) , and, recently, immunization against endogenous inhibin
Blood sample for serum human chorionic gonadotropin (HCG) was analyzed on random access immulite 2000, alpha-fetoprotein (AFP) was analyzed on ADVIA Centaur, unconjugated estriol (uE3) and Inhibin
A measured by Enzyme-Linked Immunosorbent Assay (ELISA) method by PR 4100 Micro plate Reader.
Serum levels of AMH (AMH Gen II ELISA, Beckman Coulter Inc., USA) and inhibin
B Gen II ELISA, Beckman Coulter Inc., USA) were <1.0 pmol/L (reference interval: 7.1-71.4 pmol/L) and 30.2 pg/mL (reference interval: 10-273 pg/mL), respectively.
Inhibiting the alpha subunit of inhibin
(INHA), which is related to infertility (Rah et al., 2014) and ovarian insufficiency (Li et al., 2015).
Immunohistochemical evaluation revealed diffuse positive staining with smooth muscle actin and vimentin, and negative staining with desmin, inhibin
, cytokeratin and S-100 protein in the tumor (Figure 1f).
Hyperoestrogenism reported in patients with GCT is related to tumor production of oestrogens, anti-Mullerian hormone, and inhibin
Blood work revealed elevated carbohydrate antigen (CA) 125 and positive QuantiFERON-TB Gold, but adenosine deaminase, CA 19, alpha-fetoprotein, and inhibin
B were within normal limits.
On immunohistochemistry, neoplastic cells from the large mass were positive for CD117 (Figure 2(a)), placental alkaline phosphatase (PLAP) (Figure 2(b)), and CD10 and negative for inhibin
(Figure 2(c)), cytokeratin (Figure 2(d)), [beta]-catenin, smooth muscle actin (SMA), synaptophysin, desmin, S100, [beta]-HCG, and [alpha]-fetoprotein.
The immunohistochemical markers for calretinin and inhibin
were negative (Table 1).
and other sex hormone serologies were not performed preoperatively.