DNA amplification of the 123 bp IS6110 insertion element
was carried out by two oligonucleotide primers sequence, as follows
 Hermans, P., et al., Insertion element
IS987 from Mycobacterium bovis BCG is located in a hot-spot integration region for insertion elements
in Mycobacterium tuberculosis complex strains.
Since the original publication, mcr-1 with or without the insertion element
ISAp11 has been detected on plasmids of different incompatibility groups, including IncI2, IncHI2, and IncX4, and in many different countries (1-3).
The insertion element
IS6110 has been used extensively for the detection and strain differentiation of M.
All strains had the insertion element
IS6110 detected by polymerase chain reaction as described below.
However, the heterocystous cyanobacteria have an 11 kb insertion element
present within the coding region of nifD, which is excised by site specific recombination during heterocyst differentiation.
However, this pathogen differs from the closely related dassie bacillus in its mycobacterial interspersed repetitive unit-variable number tandem repeats patterns (Table 2) by being a unique RD5das variant and by containing 21 copies of the IS6110 insertion element
(Figure 1) (compared with 10-15 copies in the dassie bacillus) (2).
Sequencing of the insertion element
(IS)26-F/R amplification product showed the location of IS26 transposase A gene (tnpA), followed by a truncated ISEcp1 mobile element upstream of the [bla.sub.CTX-M-15] gene.
A novel insertion element
from Mycobacterium avium, IS1245, is a specific target for analysis of strain relatedness.
(9.) Bauernfeind R, Benazzi S, Weiss R, Schliesser T, Willems H, Baljer G Molecular characterization of Mycobacterium paratuberculosis isolates from sheep, goats, and cattle by hybridization with a DNA probe to insertion element