F, forward; IS, insertion sequence
; Prn, pertactin; R, reverse.
In this case, a nested PCR was used to target the multi-copy IS6110 insertion sequence
, which is present in most Mycobacterium tuberculosis complex (MTBC) pathogens, these being the main causative agents of tuberculosis in humans and other animals (e.g., Muller et al., 2014).
The purpose of this study was to evaluate the efficacy of PCR tests targeting two different DNA sequences – insertion sequence
6110 (IS6110) and protein antigen b (Pab), in the same set of clinical samples from osteoarticular TB cases, and to evaluate if the sensitivity of the assay is improved.
paratuberculosis is biochemically relatively inactive and identification is currently based on mycobactin dependency and detection of the insertion sequence
IS900 by the means of the polymerase chain reaction (Collins et al 1989).
[7-12] The genetic location of [bla.sub.OXA-181] is typically on a Tn2013 transposon, immediately downstream of the insertion sequence
ISEcplB, carried on a 7.6-Kb, ColE-type plasmid.
Sequencing analysis of the ompk35 and ompk36 genes revealed the presence of the insertion sequence
IS1 between the promoter region and the start codon of the ompK35 gene, and the presence of the IS908 disrupting the ompK36 gene.
adiculatus lahorensis contains insertion sequence
(AACTGGGCTTCTCCTTAT) in the second half of V4 region (Fig.
Guzman, 2013, SNPs and an insertion sequence
in five Wx-A1 alleles as factors for variant Wx-A1 protein in wheat.
pseudomallei due to a process called reductive evolution wherein a massive invasion of many insertion sequence
(IS) elements altered the B.
We have investigated the presence and copy number of this insertion sequence
Each DD genotype was verified by a second PCR with primers specific to the insertion sequence
The assay was based on the amplification of a 181-bp fragment of the repetitive insertion sequence
I5481, which has been described in B.