An efficient protocol for callogenesis of safflower (Carthamus tinctorius L.) using leaf root and internode
explants of 21-days old in vitro grown seedlings on Murashige and Skoog (MS) basal medium were optimized by studying the influence of different growth regulators (2 4-D TIBA NAA BAP IBA) either alone or in different combinations.
Diagnosis: Eyebridge of three rows of facets, flagellomeres nodiform with paired ascoids, first flagellomere with nodal part strongly elongated, more than three times length of internode
Other morphological traits such as plant height [plant.sup.-1], number of branches [plant.sup.-1], number of leaves [plant.sup.-1], leaf area [plant.sup.-1], internode
length [plant.sup.-1], petiole length [plant.sup.-1], days to 50% flowering, number of flowers [plant.sup.-1], number of pods [plant.sup.-1], pod length, number of seeds [pod.sup.-1], days to 50% maturity, seed yield [plant.sup.-1], and 100-seed weight were also recorded at 6 months.
The cuttings of sugarcane stalks used for planting contained one node plus half of the internode
lengths above and below the node and were obtained from the middle third of sugarcane cultivars RB72454 (moderately resistant) and SP801842 (susceptible) stalks.
The growth is not restricted to one apical meristem; rather, the culm is able to grow at every internode
montana callus developed in internode
and petiole explants from juvenile trees may provide material useful for embryogenesis or indirect organogenesis, this has been successfully achieved in other forest species (25).
From the 2nd internode
, a formation of gelatinous fibers in phloem and xylem was observed, occupying half of the vascular cylinder.
Among secondary metabolites determined, the common secondary metabolites present in leaf and internode
tissue were Toluene, a-sphinosterol, Tetradecane, Pentadecane, Hexadecane, Octadecane, Hentriactone, and 9,12-Octadecadienoicacid[7,7].
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2- The herbicides treatments no effect on Sugarcane traits such as: plant height, middle internode
diametet, stem weight and stem density but 4.
a: leaf axil, b: fallen floral bract, i: internode
, s: stump.
Evaluation of promising varieties for growth, yield, quality and internode
borer incidence in South Tamil Nadu.