After 12 weeks, animals were anesthetized with ketamine (100 mg/kg) and xylazine (10 mg/kg) intraperitoneally, then rats were perfused intracardially
with 150 ml normal saline and 200 ml 4 % paraformaldehyde (PFA).
xylacine and ketamine, and intracardially
perfused with cold PBS.
Mice in the different groups were deeply anesthetized with chloral hydrate and perfused intracardially
with 30-40 ml of cold (4[degrees]C) heparinized 0.9% saline.
They were anesthetized with 70 mg/kg body weight ketamine and 7 mg/kg body weight xylazine and intracardially
perfused with 0.9% saline, followed by 4% paraformaldehyde (in phosphate buffer, pH 7.4), and then by the same fixative plus 10% sucrose.
After 2 h, the mice were intracardially
perfused with phosphate-buffered solution (PBS), and brain specimens were collected and rapidly weighed.
After the last behavioral session, rats were deeply anesthetized with an injection of sodium pentobarbital (120mg/kg, ip) and intracardially
perfused with PBS, followed by 4% paraformaldehyde.
Rats were perfused intracardially
with cold PBS containing heparin (0.2U/ml) and 4% paraformaldehyde and then stocked in 4% paraformaldehyde.
The remaining rats were anesthetized with 4% chloral hydrate and perfused intracardially
with phosphate-buffered saline (PBS) followed by 4% paraformaldehyde in PBS.
Blood samples were taken intracardially
to measure serum CK-MB levels.
Animals were anesthetized with 28% (w/v) chloral hydrate, 0.1 ml/100g of body weight, and intracardially
perfused with 4% paraformaldehyde (Sigma-Aldrich, St.
The rats were anesthetized with Nembutal (30 mg/kg) at the end of each experimental schedule, perfused intracardially
with 0.01 M phosphate-buffered saline (PBS, pH 7.4), and fixed in PLP fixative (0.01 M sodium metaperiodate; 0.075 M L-lysine-HCl; 2% paraformaldehyde, 0.03 M PB, pH 6.2).
For the measurement of biochemical parameters, blood was collected intracardially
at the time of euthanasia.