Immediately after the neurobehavioural test the animals were anaesthetized with 50 mg/kg of ketamine hydrochloride (Rotex Medica, Germany) intraperitoneally, and their thoraco-abdominal walls were dissected and intracardially
perfused with phosphate-buffered saline, and then perfusion-fixed with 10 % buffered formalin.
For histological and immunohistochemical studies, mice were intracardially
perfused with 4% paraformaldehyde (Merck, Germany) containing picric acid.
15) However, early publications suggested that bilirubin infused intracardially
to rabbits was associated with prolongation of clotting times in plasma mixed with heparin (heparin tolerance test) or after recalcification at plasma bilirubin level higher than 6 mg/dL and decreased activity of factors II and VII.
On PD 15, we intracardially
perfused the rats with phosphate-buffered saline (PBS) containing 4 percent paraformaldehyde.
The specimens were euthanized with 25% sodium thiopental injected intracardially
For detecting BrdU-labelled cells in vivo, the animals were anaesthetized and perfused intracardially
with 100 ml of heparin saline (0.
The use of potassium chloride intracardially
is still practiced in conjunction with opioids, such as fentanyl and morphine, and muscle relaxants, such as pancuronium.
5g/kg of body weight) and injected intracardially
with 7% of body weight 0.
The fate of India ink injected intracardially
into the oyster, Ostrea virginica Gmelin.
67) The issue remained dormant until a high frequency (62%) of pericardial mesotheliomas was found unexpectedly in baby Syrian Golden hamsters inoculated intracardially
with wild-type SV40.
6, 4 [degrees]C) was perfused intracardially
and followed by 4 percent paraformaldehyde in 0.
After the recordings, rats were intracardially
perfused with fixative solution and had their phrenic nerves prepared for light microscopy study.