Cells were washed twice by phosphate buffered solution (PBS) and then incubated with either isotypic
control or monoclonal antibodies against PE-CD45, FITC-CD71, FITC-CD117, APC-CD115, and APC-RANKL (receptor activator of nuclear factor [kappa]B ligand) for 30 min on ice in the dark, respectively.
space U(r,[mu]) inherits the action of [G.
We used isotypic
controls in order to check for unspecific binding and to set the markers during the evaluation of the results.
Exudate fluids were immunoprecipitated with antimouse IgG Dynal[R] magnetic beads (Invitrogen) with, as capture antibodies, 5A6G7, 01G, MEM-G/9 or G233 anti-HLA-G antibodies, W6/32 anti-pan-HLA-I antibody, or an unspecific isotypic
The mean fluorescence intensity (MFI) of the cells stained with isotypic
control antibodies was subtracted from the MFI of the cells stained with specific antibodies to get a measure of surface receptor-specific MFI.
Each specimen's event clusters were considered positive or negative when compared with the degree of the same specimen stained with the isotypic
The percentage of events reactive with each monoclonal antibody was determined, setting thresholds with isotypic
response was unexpected based upon results seen with other AIDS vaccine candidates.
1) In a model representation, isotypic
components are irreducible components, so projection operators map directly onto irreducible modules without being mixed up among multiple isomorphic copies of the same module.
control (IgGl-FITC/IgG1-PE), CD34-FITC, CD34-PE and CD34-+Rhd123 tubes were labelled using the direct method.
In an additional experiment, the primary antibody was replaced with isotypic
mouse immunoglobulin IgG2b (Sigma) at the same concentration.
n-1] (a number famous from Cayley s formula [Cay]) and that the dimension of the sign- isotypic
component is the Catalan number Cat(n, n + 1).