The control for FITC- or PE-coupled antibodies was isotypic
50 [micro]l EMP suspension was incubated with 3 [micro]l of anti-human CD31-FITC (557508, BD, USA) or 3 [micro]l of isotypic
immunoglobulins IgG1-FITC (551954, BD, USA).
Both structures are isotypic
with very related geometric parameters.
Mouse monoclonal antiactive [beta]-catenin (ABC) which is specific against nuclear [beta]-catenin, and goat anti-mouse IgG-rhodamine were obtained from Millipore (Massachusetts, USA), whereas rabbit polyclonal anti-cleaved caspase-3 antibody was from Calbiochem (Massachusetts, USA), EMD Chemicals, goat anti-rabbit IgG-FITC, rabbit anti-goat IgGHRP conjugated and IgG1 mouse isotypic
control were from Vector Laboratories (Peterborough, UK).
FACopyrightrey, Different Adsorption Behaviors of Methane and Carbon Dioxide in the Isotypic
Nanoporous Metal Terephthalates MIL-53 and MIL-47, J.
Cells were washed twice by phosphate buffered solution (PBS) and then incubated with either isotypic
control or monoclonal antibodies against PE-CD45, FITC-CD71, FITC-CD117, APC-CD115, and APC-RANKL (receptor activator of nuclear factor [kappa]B ligand) for 30 min on ice in the dark, respectively.
This is not the case of planar systems; on the contrary, it simplifies the analysis because of the reduction to isotypic
Results were expressed as the percentage difference compared with isotypic
control (IC) using the formula [mean fluorescence intensity (MFI) of sample-MFI of IC]/MFI of IC.
Fluorescence labeled liposomes were prepared with anti-EGFR antibodies or isotypic
human immunoglobulins coupled with the DSPE-PEG2000Mal linker.
controls were also used in all experiments, including mouse IgG1 (679.1Mc7) and IgG2a (UCTH-1) labeled with FITC and PE, respectively.
space U(r,[mu]) inherits the action of [G.sub.r] and therefore decomposes as