CHICAGO -- Compared with that of calcineurin inhibitors, belatacept appears to be associated with a lower risk of keratinocyte
carcinomas in solid organ transplant patients, based on results from a single-center analysis presented at the annual meeting of the Society for Investigative Dermatology.
Beyond their relevance in thermoregulation and sensory functions, hair follicles play an important role in wound healing, as they are a supplementary source of keratinocyte
stem cells (Fuchs, 2016).
The isolated keratinocytes
were cultivated in a serum-free keratinocyte
growth medium (K-SFM, Gibco), and fibroblasts were cultivated in Dulbecco's Modified Eagle's Medium (DMEM) (HyClone, UT, USA) supplemented with 10% Fetal Calf Serum (FCS, Gibco, Canada).
HaCaT cells, human spontaneously immortalized keratinocytes
with full epidermal differentiation capacity, have been widely used as an in vitro keratinocyte
axis inhibits keratinocyte
survival and proliferation.
In addition to necrosis of keratinocytes
, the tissue damage in SJS/TEN is also characterized by widespread keratinocyte
apoptosis mediated by interactions between the death receptor Fas and its ligand Fas L and by the cytotoxic protein granulysin secreted by activated T cells and NK cells [19, 32, 33, 52, 54, 55].
HaCaT cells, spontaneously immortalized human keratinocyte
line , were kindly provided by Cell Line Service GmbH (Eppelheim, Germany) and cultured in 5% C[O.sub.2] at 37[degrees]C in regular Dulbecco's Modified Eagle's Medium (DMEM) (Euroclone S.P.A., Milan, Italy) containing 1.8 mM [Ca.sup.2+], or with DMEM (Gibco, Life Technologies, Carlsbad, CA, USA) at low concentration of [Ca.sup.2+] (0.07 mM).
Research has proven that smoking 1) causes a decrease in capillary blood flow to the skin caused by vasoconstriction, 2) releases an abundance of free radicals into the skin tissues, 3) increases keratinocyte
dysplasia, 4) decreases keratinocyte
migration, and 5) decreases the production of erythrocytes (red blood cells).
is the predominant cell type in the epidermis, which is outermost layer of the skin.
Evidence that cadherins play a role in the downregulation of integrin expression that occurs during keratinocyte
In contrast, an inhibitory effect of [beta]-catenin on reepithelialization has been suggested in a study that showed enhanced epidermal nuclear accumulation of [beta]-catenin at the edge of chronic ulcers and that pharmacological stabilization of [beta]-catenin inhibits keratinocyte
migration by increasing c-Myc expression .