First detection of Leishmania infantum kinetoplast
DNA in hair of wild mammals: application of qPCR method to determine potential parasite reservoirs.
Also we have already assessed the sensitivity of two methods of semi-nested for minicircle kinetoplast
(kDNA) and nested PCR of ITS-rDNA to detect Leishmania parasites in Phlebotomus papatasi which revealed the high infection rates of L.
A multiple genetic marker approach (mini-circle kinetoplast
DNA, restriction analysis of 3'UTR of HSP70 and HSP70 gene sequence analysis) was adopted (10) for the same.
Ziyaeyan, "Detection of Leishmania infantum kinetoplast
DNA in the whole blood of asymptomatic individuals by PCR-ELISA and comparison with other infection markers in endemic areas, southern Iran," The American Journal of Tropical Medicine and Hygiene, vol.
is a diagnostic structure of the Kinetoplastida order, which encompasses the Trypanosomatidae family.
Crithidia lucilia is a hemoflagellate protozoa that has an organelle called a kinetoplast
which contains a high-concentration native double-stranded DNA.
After 1 h at room temperature, we rinsed the slides in PBS, stained the nuclear and kinetoplast
DNA with DAPI, and mounted them with Vecta-Shield medium (Vector Laboratories).
Luteolin, an abundant dietary component is a potent anti-leishmanial agent that acts by inducing topoisomerase II mediated kinetoplast
DNA cleavage leading to apoptosis.
, and cytoplasmic limit) was observed.
Oliveira et al., "Kinetoplast
DNA signatures of Trypanosoma cruzi strains obtained directly from infected tissues," American Journal of Pathology, vol.
Further insight into the role of Top II-induced DSBs in mitochondria maybe drawn from parallels in Kinetoplastids, a group of flagellated protozoa which includes the parasitic Trypanosoma and Leishmania species, which possess a characteristic dense granule of DNA called a kinetoplast
within their single mitochondrion.