Leishmania-specific PCR amplification produced positive amplicons from kinetoplast
minicircle, genomic heat shock protein 70, and internal transcribed spacer 1 (ITS1) targets.
Rounded and oval structures with kinetoplast
, phagocytosed in macrophages were observed.
, and cytoplasmic limit) was observed.
The present study was designed to report the prevalenceof Trypanosoma brucei in blood samples of donkeys (n=283) collected from Dera Ghazi Khan District in Punjab (Pakistan) through PCR amplification of their maxicircle kinetoplast
DNA (kDNA) during four seasons in 2013 and to demonstrate the effect of this parasite on complete blood count (CBC) and selected parameters of serum.
Luteolin, an abundant dietary component is a potent anti-leishmanial agent that acts by inducing topoisomerase II-mediated kinetoplast
DNA cleavage leading to apoptosis.
4,18-22) The target DNAs for these studies have been kinetoplast
DNA, rDNA (internal transcribed spacer [ITS]), repetitive nuclear DNA, mini-exon genes, and microsatellite DNA.
protozoa are characterized by a single mitochondrion near the flagellum which contains DNA in a small compartment called a kinetoplast
In addition, ultrastructural analyses showed remarkable alterations in the morphology of the Golgi complex, kinetoplast
, endoplasmic reticulum and plasma membrane of trypomastigotes, confirming a pattern consistent with necrotic cell death.
and Trypanosoma cruzi, and is characterized by the presence of a kinetoplast
(Shapiro and Englund, 1995).
The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast
DNA OligoC-TesT for Diagnosis of Chagas Disease: A Multi-cohort Comparative Evaluation Study.
Electronic microscopy transmission studies with cells treated with pentamidine showed condensation and disruption of kinetoplast
DNA core and collapse of mitochondrial membrane [39, 40].
First detection of Leishmania major in peridomestic Phlebotomus papatasi from Isfahan province, Iran: Comparison of nested PCR of nuclear ITS ribosomal DNA and semi-nested PCR of minicircle kinetoplast