However, these networks occupy kinetoplasts of different shapes and dimensions.
In recent years, AFM has been used to study kDNA topology and the effect of drugs on the kinetoplasts of trypanosomatids.
of parasites were subterminal, the undulating membranes were well developed and there was a substaintial free flagellum.
Anti-dsDNA was defined as positive only when fluorescence staining was detected in kinetoplasts
of Crithidia luciliae.
Kinetoplasts play an important role in the drug responses of Trypanosoma brucei.
On the basis of ultrastructural morphology and sequence comparison of small subunit ribosomal RNAs, Leishmania and Trypanosoma are phylogenetically related and both species have a kinetoplast that contains superhelical DNA (9).
The central scale was removed from one of the lesions, and scrapings from the base of the lesion were stained with Giemsa stain, which showed intracellular amastigotes with peripheral nuclei and rod-shaped kinetoplasts
. Results of indirect immunoflorescent antibody (IFA) testing were positive for Leishmania parasites (titer 6,400).
The binding of antihistone antibodies to crithidia luciliae kinetoplasts
is growth cycle-dependent.
Histopathologic examination of hematoxylin-eosin-stained tissue revealed a dermal granulomatous inflammation with a predominantly lymphoid infiltrate (Figure 2) and numerous, small (2-4 [micro]m in diameter), oval to round organisms with bar-shaped paranuclear kinetoplasts, morphologically consistent with leishmanial parasites (Figure 3).
However, leishmanial parasites contain a characteristic bar-shaped paranuclear kinetoplast. (5)
(8) Leishmania species amastigotes are round and exhibit paranuclear bodies (kinetoplasts
Cytologically, the cytoplasm of a typical Leishman-Donovan body contains a nucleus and a kinetoplast
. (1) The complete form of a Leishman-Donovan body can be seen in aspiration of bone marrow, lymph nodes, and skin smears.