isolated from apple rhizosphere Parameters Fluorescent Pseudomonas isolates M1 M2 SH1 SO1 DE-18 Catalase test + + + + + Gelatin liquification + + + + - Lecithinase
activity + + - - - Oxidase test + + + + + Spore staining - - - - - Starh hydrolysis - - - - - Tween 80 hydrolysis - - - - - Denitrification + + + + + Growth at 4[degrees]C + + + - + Growth at 41[degres]C - + - + - (-) Indicates negativity of test (+) indicates positivity of test Table 4.
9 % Similarity (%) Table 2: characteristics of B2 isolate from contaminated soil B2 + GRAM - oxidase + Catalase Facultative anaerobe Respiration + Motility - NO3 + GLU (fermentation) - ADH - URE + GEL + ONPG - ARA + MAN - LDC - ODC - TDA - IND - VP - INO - CIT - SOR - RHA - SAC - MEL - AMY - H2S Gamma hemolvsis on blood - Amvlase - Lecithinase
+ Caseine + Snore formina Brevibacillus laterosporus Tentative identity 94.
The isolated bacteria are motile, rod shape and Gram positive, which produced acid from glucose, had catalase activity, reduced nitrate, showed positive lecithinase
and indoles tests, could not hydrolyze starch, and had negative mannitol and VP tests.
Preliminary isolation, identification and selection of strains of lactobacilli that do not exhibit hemolytic, cytotoxic, catalase, lecithinase
, urease, gelatinase, RNA-ase, DNA-ase activity allowed to use them as promising strains to create a functional food for the rehabilitation of cancer patients.
The positive colonies were subjected to biochemical test for identification such as catalase, coagulase, oxidase, indole, methyl red, Voges-Proskauer, Citrate, TSI, stormy clot fermentation, Lecithinase
, CAMP tests and sugar fermentation reaction as per Barrow and Feltham (1993).
So Fairbairn used the name LPL instead of lecithinase
B, which was proposed by Contardi and Ercoli.
Agar diffusion tests made on mediums supplemented with different fat sources showed that all strains don't have lecithinase
activity, neither lipolytic activity.
, collagenase), which lead to the digestion of fascial barriers, thus fueling the rapid extension of the infection.
Results showed that all the isolates (VP1-VP3 and VR1-VR3) produced P-hemolysis on rabbit blood agar, lecithinase
, proteinase, and gelatinase, but DNase and lipase were not produced.
botulinum was identified and differentiated from other botulinum toxin-producing clostridia on the basis of Gram stain reaction, morphologic features, motility, lipase and lecithinase
reactions on egg yolk agar, sugar fermentations, esculin hydrolyis, gelatin liquefaction, and production of botulinum neurotoxin.
The organisms also grew on chocolate agar and were positive for catalase and lecithinase
Biochemical and morphological characterization: The microbiological identification of the isolates was done by: Gram, lecithinase
and haemolytic activity, screening for presence of parasporal bodies by phase-contrast microscopy.