It could grow on media containing 0.001% lysozyme and showed strong lecithinase
and hemolytic activity with blood agar test.
Gulve found that out of 90 Actinomycetes 76 (84.44%), 70 (77.78%), 65 (72.22%), 39 (43.33%), 34 (37.78%) and 15 (16.67%) number of Actinomycetes were possessing protease, gelatinase, amylase, lecithinase
, cellulase and urease activity respectively (56).
Further the isolates were grown in egg-yolk agar and Perfringens agar for detection of lecithinase
production and confirmation.
aureus based on their colony morphology (yellow colonies and a surrounding yellow medium in mannitol salt agar), tellurite reduction, lecithinase
activity, and mannitol fermentation and by their ability to coagulate human plasma (tube coagulase test) .
Table 1: characteristics of the PI isolate from contaminated soil P1 - GRAM + oxidase + Catalase Obligate aerobe Respiration + Motility + KingA + kingB - Mcc + NO3 - TRP - GLU (fermentation) + ADH + URE - ESC + GEL - PNPG + GLU - ARA - MNE + MAN + NAG - MAL + GNT + CAP + ADI + MLT + CIT - PAC Pseudomonas aerupinosa Tentative identib 99.9 % Similarity (%) Table 2: characteristics of B2 isolate from contaminated soil B2 + GRAM - oxidase + Catalase Facultative anaerobe Respiration + Motility - NO3 + GLU (fermentation) - ADH - URE + GEL + ONPG - ARA + MAN - LDC - ODC - TDA - IND - VP - INO - CIT - SOR - RHA - SAC - MEL - AMY - H2S Gamma hemolvsis on blood - Amvlase - Lecithinase
+ Caseine + Snore formina Brevibacillus laterosporus Tentative identity 94.1 % Similarity (%)
Preliminary isolation, identification and selection of strains of lactobacilli that do not exhibit hemolytic, cytotoxic, catalase, lecithinase
, urease, gelatinase, RNA-ase, DNA-ase activity allowed to use them as promising strains to create a functional food for the rehabilitation of cancer patients.
Under our experimental conditions, the Klebsiella isolates from the enteral diets we tested did not exhibit aerobactin production (Figure 1), hemolytic activity, or lecithinase
So Fairbairn used the name LPL instead of lecithinase
B, which was proposed by Contardi and Ercoli.
Agar diffusion tests made on mediums supplemented with different fat sources showed that all strains don't have lecithinase
activity, neither lipolytic activity.
The latter microorganisms, in turn, may produce enzymes (e.g., lecithinase
, collagenase), which lead to the digestion of fascial barriers, thus fueling the rapid extension of the infection.
Results showed that all the isolates (VP1-VP3 and VR1-VR3) produced P-hemolysis on rabbit blood agar, lecithinase
, proteinase, and gelatinase, but DNase and lipase were not produced.
Test Result Cell shape Rods Gram stain +ve Spore formation +ve Growth on MYP Non-mannitol utilizer Motility +ve Growth at pH 6-8 +ve Growth at 50[degrees]C +ve Growth at 7.5% NaCl -ve Starch hydrolysis +ve Casein hydrolysis +ve Tween 20 hydrolysis -ve Nitrate reduction +ve Catalase +ve Oxidase +ve Urease -ve Indole production -ve Citrate utilization -ve Voges-Proskauer (VP) +ve Methyl red (MR) -ve Gelatin liquefaction +ve Hemolysis on sheep blood agar [beta]-Hemolytic Lecithinase
on MYP +ve Penicillin resistance +ve Oxidative fermentation (OF): Glucose fermentation +ve (yellow/blue: O/NF) * Galactose fermentation +ve (yellow/blue: O/NF) Sucrose fermentation +ve (yellow/yellow: O/F) ** * O/NF: oxidative/non-fermentative.