The MDA-MB-231 and SW579 cells of lentivirus
infected or afatinib treated were collected and stored in a 75% alcohol-PBS solution for flow cytometry analysis.
Key word: bovine LXR[alpha] gene, lentivirus
, vector construction, gene silencing.
Abstract.--Equine Infectious Anemia Virus (EIAV) is a simple, macrophage-tropic lentivirus
that infects members of the family Equidae.
They delivered the factors into the cells with a lentivirus
, as used in some forms of gene therapy.
Meanwhile, Pin1 shRNA was used to knock down Pin1 protein in cultured neurons; the level of Pin1 protein was decreased by about 82% after neurons were transfected by Pin1 shRNA lentivirus
for 48 h (Figure 4(b)).
Louis, MO, 100ng/mL) and/ or TREM2 lentivirus
To construct a proinsulin (Pro-INS) overexpressing cell line, the human Pro-INS open reading frame (GenBank number, NM_000207) was synthesized and recombined into the lentivirus
VEGF protein expression was then investigated using western blotting, which showed reduced VEGF protein expression in HTF cells transfected with lentivirus
VEGF-shRNA (Figure 2).
After transduction with the lentivirus
, cells were stained with DAPI to visualize the nucleus.
In order to upregulate hsa-miR-200a expression, hsa-miR-200a mimic lentivirus
and a nonspecific control lentivirus
were purchased from the Biological Technology Company (GenePharma, Shanghai, China).
Caprine arthritis encephalitis virus (CAEV) is a lentivirus
that is closely related to sheep Meadi-Visna virus.
Goettingen, Germany, September 23, 2016 --(PR.com)-- Lentivirus
is an enveloped, single-stranded RNA virus from the Retroviridae family capable of infecting both dividing and non-dividing cells.