The E3 ubiquitin ligase
neural precursor cell expressed developmentally down-regulated 4-1 (NEDD4-1) negatively regulates PTEN protein stability through polyubiquitination.
To test whether Hbo1 alone has ubiquitin E3 ligase
activity, an array of E2 ubiquitin conjugating enzymes for self-ubiquitination of commercial Hbo1 protein were screened by mixing commercial recombinant Hbo1 protein with E1, E2, ATP, and biotinylated ubiquitin and probing with Cy5-conjugated streptavidin (Fig.
Simple modular architecture research tool (SMART) analysis revealed that horse GCLC protein contains the gammaglutamylcysteine synthetase (GCS) domain from amino acid position 236 to 608, which represents a signature catalytic subunit of glutamate-cysteine ligase
domain (EC 6.
This study may explain why some cancer predisposing mutations are found in the front part of the BRCA1 gene - the part that allows it to function as a ubiquitin ligase
In addition, UCHL1 can form dimers, whose form seems to act as another enzymatic activity in UCHL1, the ubiquitin ligase
Finally, DNA ligase
catalyses the formation of two new phosphodiester bonds and thus seals the sugar-phosphate backbone.
Some sumoylation assays revealed that in the presence of E1 and E2, the E3 ligase
was dispensable to accomplish SUMO conjugation.
Briefly, the 8 epialleles were interrogated individually in 10-[micro]-L reactions, each consisting of 5 ng of PCR product, 2 U of ligase
, 100 nmol/L of an epiallele probe, and 50 nmol/L of the reporter probe labeled with the TYE665 fluorescent dye.
Patsi will oversee the current portfolio in EMEA as well as international efforts for the company's pipeline of clinical programs in areas including immunotherapy, epigenetics, ubiquitin ligase
targets, cancer metabolism and others.
Using sophisticated gene-targeting techniques to disable particular genes in human cells and then studying the impact on telomere fusion, the researchers found that cells escaped death only when the gene Ligase
3 was active but not when its action, which appears to promote fusion within like chromosomes rather than between different chromosomes, was blocked.
One [mu]l of 10xligation buffer and 1 p1 of Ligase
were added to the mixture adjusting the final volume to 10 [mu]1 with dd[H.
Inhibitors of apoptosis (IAP) proteins regulate cancer cell survival through their RING domain ubiquitin ligase