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 (lī′gās′, -gāz′)
Any of a class of enzymes that catalyze the linkage of two large molecules.

[Latin ligāre, to bind; see leig- in Indo-European roots + -ase.]


(Biochemistry) any of a class of enzymes that catalyse the formation of covalent bonds and are important in the synthesis and repair of biological molecules, such as DNA


(ˈlaɪ geɪs, -geɪz)

an enzyme that catalyzes the joining of two molecules by forming a covalent bond accompanied by the hydrolysis of ATP. Also called synthetase.
[1961; < Latin lig(āre) to tie, bind]
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References in periodicals archive ?
The E3 ubiquitin ligase neural precursor cell expressed developmentally down-regulated 4-1 (NEDD4-1) negatively regulates PTEN protein stability through polyubiquitination.
To test whether Hbo1 alone has ubiquitin E3 ligase activity, an array of E2 ubiquitin conjugating enzymes for self-ubiquitination of commercial Hbo1 protein were screened by mixing commercial recombinant Hbo1 protein with E1, E2, ATP, and biotinylated ubiquitin and probing with Cy5-conjugated streptavidin (Fig.
Simple modular architecture research tool (SMART) analysis revealed that horse GCLC protein contains the gammaglutamylcysteine synthetase (GCS) domain from amino acid position 236 to 608, which represents a signature catalytic subunit of glutamate-cysteine ligase domain (EC 6.
This study may explain why some cancer predisposing mutations are found in the front part of the BRCA1 gene - the part that allows it to function as a ubiquitin ligase.
In addition, UCHL1 can form dimers, whose form seems to act as another enzymatic activity in UCHL1, the ubiquitin ligase activity
Finally, DNA ligase catalyses the formation of two new phosphodiester bonds and thus seals the sugar-phosphate backbone.
Some sumoylation assays revealed that in the presence of E1 and E2, the E3 ligase was dispensable to accomplish SUMO conjugation.
Briefly, the 8 epialleles were interrogated individually in 10-[micro]-L reactions, each consisting of 5 ng of PCR product, 2 U of ligase, 100 nmol/L of an epiallele probe, and 50 nmol/L of the reporter probe labeled with the TYE665 fluorescent dye.
Patsi will oversee the current portfolio in EMEA as well as international efforts for the company's pipeline of clinical programs in areas including immunotherapy, epigenetics, ubiquitin ligase targets, cancer metabolism and others.
Using sophisticated gene-targeting techniques to disable particular genes in human cells and then studying the impact on telomere fusion, the researchers found that cells escaped death only when the gene Ligase 3 was active but not when its action, which appears to promote fusion within like chromosomes rather than between different chromosomes, was blocked.
One [mu]l of 10xligation buffer and 1 p1 of Ligase were added to the mixture adjusting the final volume to 10 [mu]1 with dd[H.
Inhibitors of apoptosis (IAP) proteins regulate cancer cell survival through their RING domain ubiquitin ligase activity.