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Thus, the analytical sensitivity of our method is approximately 6 copies of input DNA, about 165 000-fold, 35-fold, and 200-fold lower than closed-tube colorimetric PCR (15), loop mediated isothermal amplification (14), and ligase chain reaction (11, 16), respectively.
Homogeneous and sensitive detection of microRNA with ligase chain reaction and lambda exonuclease-assisted cationic conjugated polymer biosensing.
Reproducibility issues have been noted with ligase chain reaction amplification.
In three states, publicly funded screening programs switched from gonorrhea culture to nonculture tests; health departments in Iowa and Mississippi switched to using nucleic acid probe assays, and in North Dakota to ligase chain reaction tests.
Ligases have been used to amplify single-DNA base changes with the ligase chain reaction (LCR) and ligase detection reaction (LDR) assays (25, 26).
We'll carry on in that vein this month with an examination of two other methodologies used in some clinical settings: the ligase chain reaction (LCR) and helicase dependant amplification (HDA).
Results of tests for Neisseria gonorrhoeae and Chlamydia trachomatis by ligase chain reaction (LCx; Abbot Laboratories, Vienna, Austria) were negative.
Most larger medical labs offer one of four licensed urine, cervical, and urethral amplified gonorrhea and chlamydia tests: polymerase chain reaction, ligase chain reaction, transcription-mediated amplification, or strand displacement amplification.
Ligase chain reaction assays were used to detect gonococcus and chlamydia in the urine og the 579 respondents who complied.